Aconitum bucovinense Zapał. is the European species of monkshood, endemic to the Eastern and Southern Carpathians. A protocol has been developed for the in vitro regeneration of adventitious shoots by indirect organogenesis from leaf explants. An initiation of cultures carried out on a medium (B5 macronutrients, MS micronutrients) with picloram and kinetin allowed obtaining a callus. More than a 200% FM increase of the callus and at the same time differentiation of adventitious buds were obtained on IBA and BAP supplemented medium. Excised buds were used to establish shoot cultures and multiplied after a transfer to nutrient media with an addition of BAP with IBA, IAA or NAA. Almost 70% of rooted shoots were obtained on a 1.5 mg L⁻¹ IBA and 1.0 mg L⁻¹ BAP supplemented medium with simultaneous efficient multiplication. An analysis of peroxidase activity revealed its gradual increase in shoots until the appearance of roots. For the first time, an efficient way to regenerate, multiply and root A. bucovinense shoots has been developed and can be used for ex situ conservation of this species.

乌头 bucovinense Zapał。是欧洲的附子物种，是东部和南部喀尔巴阡山脉的特有种。已经开发了一种通过叶外植体的间接器官发生体外再生不定芽的方案。在含有毒莠定和激动素的培养基（B5 宏量营养素、MS 微量营养素）上开始培养允许获得愈伤组织。在 IBA 和 BAP 补充培养基上，愈伤组织的 FM 增加超过 200%，同时不定芽的分化。切除的芽用于建立枝条培养物，并在转移到营养培养基中添加 BAP 和 IBA、IAA 或 NAA 后繁殖。^{在 1.5 mg L -1} IBA 和 1.0 mg L ^-1上获得了几乎 70% 的生根芽BAP 补充了具有同时有效增殖的培养基。对过氧化物酶活性的分析显示其在芽中逐渐增加，直到出现根。第一次，一种有效的方法来重新生成、乘法和根A。bucovinense芽已经开发出来，可用于该物种的异地保护。