牡蛎（Crassostrea gigas）多糖通过胆汁酸-FXR-AMPKα途径改善高脂饮食诱导的肝脏氧化应激和炎症,Journal of Agricultural and Food Chemistry

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牡蛎（Crassostrea gigas）多糖通过胆汁酸-FXR-AMPKα途径改善高脂饮食诱导的肝脏氧化应激和炎症
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-07-07 , DOI: 10.1021/acs.jafc.2c02490
Yuyang Ma ₁ , Xue Liu ₁ , Defu Liu ₁ , Zihao Yin ₁ , Xinyi Yang ₁ , Mingyong Zeng _{1,

2}

Affiliation

牡蛎多糖（OPS）具有多种生物活性。在这项研究中，我们旨在研究 OPS 改善高脂饮食 (HFD) 诱导的小鼠肝脏氧化应激和炎症的潜在机制。结果表明，OPS降低了HFD诱导的血清转氨酶水平升高，并减轻了肝脏氧化应激和炎症。此外，OPS 调节胆汁酸代谢并增加肝脏、血清和粪便中的胆汁酸含量。血清胆汁酸谱结果表明，OPS 降低了与法尼醇 X 受体 (FXR) 的高亲和力激动剂相关的鹅去氧胆酸、脱氧胆酸和石胆酸的水平。Western印迹分析表明，OPS通过下调肝脏FXR表达并促进其下游CYP7A1、CYP27A1、和 CYP8B1 蛋白表达。同时，OPS 通过调节 FXR-AMPKα-Nrf2/NF-κB 信号传导以减少 p-IκBα/IκBα、p-NF-κB p65/NF-κB p65、IL-1β 和 TNF- 来改善肝脏中的氧化应激和炎症。 α 表达并增加 p-Nrf2/Nrf2、HO-1 和 NQO-1 的表达。本研究首次从胆汁酸代谢的角度探讨了OPS改善肝脏氧化应激和炎症的可能机制，为OPS作为功能性食品的新来源提供了理论依据。

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Oyster (Crassostrea gigas) Polysaccharide Ameliorates High-Fat-Diet-Induced Oxidative Stress and Inflammation in the Liver via the Bile Acid-FXR-AMPKα Pathway

Oyster polysaccharides (OPS) have a variety of biological activities. In this study, we aimed to investigate the potential mechanisms of OPS to ameliorate hepatic oxidative stress and inflammation in mice induced by a high-fat diet (HFD). The results showed that OPS reduced the HFD-induced increases in serum transaminase levels and alleviated hepatic oxidative stress and inflammation. Moreover, OPS regulated bile acid metabolism and increased bile acid content in the liver, serum, and feces. Serum bile acid profile results indicated that OPS reduced levels of chenodeoxycholic acid, deoxycholic acid, and lithocholic acid associated with high-affinity agonists of Farnesol X receptor (FXR). Western blot analysis showed that OPS accelerated bile acid metabolism by downregulating hepatic FXR expression and promoting its downstream CYP7A1, CYP27A1, and CYP8B1 protein expression. Meanwhile, OPS ameliorated oxidative stress and inflammation in the liver by modulating FXR-AMPKα-Nrf2/NF-κB signaling to reduce p-IκBα/IκBα, p-NF-κB p65/NF-κB p65, IL-1β, and TNF-α expression and increase p-Nrf2/Nrf2, HO-1, and NQO-1 expression. This study was the first to explore the possible mechanism of OPS in improving liver oxidative stress and inflammation from the perspective of bile acid metabolism, providing a theoretical basis for OPS as a new source of functional food.

更新日期：2022-07-07

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