Rugose spiralling whitefly, Aleurodicus rugioperculatus Martin (Hemiptera: Aleyrodidae) is an introduced phloem feeder of guava. Gut symbiotic bacteria alter the physiology of the host insect. The present study characterized the nine bacterial isolates from the second nymphal stage of rugose spiralling whitefly using seven different bacteria growth media viz., Nutrient Agar (NA), Luria Bertani agar (LBA), MacConkey agar (MCA), Tryptic Soya Agar (TSA), Endo Agar (EA), R2A agar and MRS agar. Among the nine bacterial isolates, chitinase-producing isolates (seven species) were proceeded for biochemical tests, protease and siderophore activities. Out of seven chitinase producers, protease (seven species) and siderophore (two species) producers were identified using skim milk agar and Chromazurol S (CAS) agar plates, respectively. The biochemical tests showed that all bacterial isolates were Gram positive. Bacterial isolates SGRSW2, SGRSW4, SGRSW6 and SGRSW8 showed negative for carbohydrate fermentation, indole production, citrate utilization, starch hydrolysis tests and also, SGRSW4 showed positive for lactose fermentation. 16S rRNA gene sequencing results revealed that Bacillus genus alone is associated with rugose spiralling whitefly which includes Bacillus albus, Bacillus tequilensis and Bacillus altitudinis. Bacterial communities associated with rugose spiralling whitefly offers basic knowledge about their functional significance to study the physiological role in the host insect.

皱纹螺旋粉虱，Aleurodicus rugioperculatusMartin（半翅目：Aleyrodidae）是一种引进的番石榴韧皮部饲养者。肠道共生细菌会改变宿主昆虫的生理机能。本研究使用七种不同的细菌生长培养基，即营养琼脂 (NA)、Luria Bertani 琼脂 (LBA)、麦康凯琼脂 (MCA)、胰蛋白酶大豆琼脂 (TSA) 表征了来自皱褶螺旋粉虱第二若虫阶段的九种细菌分离物。 )、远藤琼脂 (EA)、R2A 琼脂和 MRS 琼脂。在九个细菌分离物中，对产生几丁质酶的分离物（七种）进行了生化测试、蛋白酶和铁载体活性测试。在七个几丁质酶生产者中，蛋白酶（七个物种）和铁载体（两个物种）生产者分别使用脱脂牛奶琼脂和 Chromazurol S (CAS) 琼脂平板进行鉴定。生化测试显示所有细菌分离物均为革兰氏阳性。细菌分离株SGRSW2、SGRSW4、SGRSW6和SGRSW8对碳水化合物发酵、吲哚产生、柠檬酸盐利用、淀粉水解试验呈阴性，SGRSW4对乳糖发酵呈阳性。16S rRNA 基因测序结果显示，单独的芽孢杆菌属与有皱纹的螺旋粉虱有关，其中包括白芽孢杆菌、tequilensis芽孢杆菌和高地芽孢杆菌。与褶皱螺旋粉虱相关的细菌群落提供了有关其功能意义的基本知识，以研究寄主昆虫的生理作用。