The purpose of this study is to examine the association between G protein-coupled receptor 87 (GPR87) and lung adenocarcinoma (LUAD) metastasis and immune infiltration. The Cancer Genome Atlas (TCGA) and Gene Expression Omnibus (GEO) datasets extract clinical data. According to the TCGA database, increased GPR87 expression predicts poor overall survival, progression-free interval, and disease-specific survival in LUAD patients. The meta-analysis also reveals a significant association between high GPR87 expression and poor overall survival. Moreover, functional experiments demonstrate that GPR87 silencing reduces LUAD cell invasion and migration. Immunoblotting shows that GPR87 knockdown decreased Vimentin and N-cadherin expression and increased E-cadherin expression in LUAD cells. GPR87 expression in LUAD is positively correlated with immune infiltration. In addition, GPR87 expression is associated with immune and chemotherapy resistance in LUAD patients. Our findings indicate that GPR87 promotes tumor progression and is correlated with immune infiltration, suggesting GPR87 as a possible biomarker for prognosis prediction in LUAD.

本研究的目的是检查 G 蛋白偶联受体 87 (GPR87) 与肺腺癌 (LUAD) 转移和免疫浸润之间的关联。癌症基因组图谱 (TCGA) 和基因表达综合 (GEO) 数据集提取临床数据。根据 TCGA 数据库，GPR87表达增加预示着 LUAD 患者的总生存期、无进展间隔期和疾病特异性生存期较差。荟萃分析还揭示了高GPR87表达与较差的总体生存率之间的显着关联。此外，功能实验表明GPR87沉默减少了LUAD细胞的侵袭和迁移。免疫印迹显示 GPR87 敲低降低了 LUAD 细胞中波形蛋白和 N-钙粘蛋白的表达并增加了 E-钙粘蛋白的表达。LUAD中 GPR87 的表达与免疫浸润呈正相关。此外，GPR87表达与 LUAD 患者的免疫和化疗耐药性有关。我们的研究结果表明，GPR87促进肿瘤进展并与免疫浸润相关，表明GPR87作为 LUAD 预后预测的可能生物标志物。