The antifungal mechanisms of plant volatile compound 1-octanol against Aspergillus flavus growth,Applied Microbiology and Biotechnology

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The antifungal mechanisms of plant volatile compound 1-octanol against Aspergillus flavus growth
Applied Microbiology and Biotechnology ( IF 3.9 ) Pub Date : 2022-07-02 , DOI: 10.1007/s00253-022-12049-z
Yu-Liang Qin _{1,

2} , Shuai-Bing Zhang _{1,

2} , Yang-Yong Lv _{1,

2} , Huan-Chen Zhai _{1,

2} , Yuan-Sen Hu _{1,

2} , Jing-Ping Cai _{1,

2}

Affiliation

Abstract

The exploitation of active ingredients from plant volatile organic compounds as natural gaseous fungicides shows remarkable potential for controlling fungal decay in postharvest agroproducts. Although 1-octanol is a common component of cereal volatiles, its antifungal potency against spoilage fungi in postharvest grains remains unclear. In this study, we studied the effectiveness of 1-octanol against Aspergillus flavus growth in postharvest grains and its mechanisms of action. 1-Octanol vapor and liquid contact dose-dependently inhibited A. flavus spore germination and mycelial growth at a low concentration. The simulated storage experiment demonstrated that 300 μL/L of 1-octanol vapor completely controlled A. flavus growth in wheat, corn, and paddy grains with 20% moisture content. 1-Octanol treatment irreversibly damaged the conidial and mycelial morphology of A. flavus and caused electrolyte leakage due to reduced plasma membrane integrity. It induced apoptosis along with morphological abnormalities, phosphatidylserine externalization, mitochondrial membrane potential depolarization, intracellular reactive oxygen species accumulation, and DNA fragmentation in A. flavus cells. Metabolomic analysis revealed that 1-octanol treatment disrupted the biosynthesis of unsaturated fatty acids, ATP-binding cassette transporters, amino acid metabolism, and glycerophospholipid metabolism. This study demonstrated the promising application potential of 1-octanol as a biofumigant for preventing fungal spoilage of postharvest cereal grains.

Key points

• (1) 1-Octanol inhibits Aspergillus flavus growth in the vapor phase and liquid contact;

• (2) 1-Octanol damages membrane integrity and induces apoptosis of A. flavus;

• (3) Metabolomic changes in A. flavus mycelia were analyzed after 1-octanol treatment.

中文翻译：

植物挥发性化合物1-辛醇对黄曲霉生长的抗真菌机制

摘要

将植物挥发性有机化合物中的活性成分用作天然气杀菌剂，在控制采后农产品中的真菌腐烂方面显示出巨大的潜力。虽然 1-辛醇是谷物挥发物的常见成分，但其对收获后谷物中腐败真菌的抗真菌效力仍不清楚。在这项研究中，我们研究了 1-辛醇对收获后谷物中黄曲霉生长的有效性及其作用机制。1-辛醇蒸气和液体接触在低浓度下剂量依赖性地抑制A. flavus孢子萌发和菌丝体生长。模拟贮藏实验表明，300 μL/L 1-辛醇蒸气完全控制黄曲霉在水分含量为 20% 的小麦、玉米和稻谷中生长。1-辛醇处理不可逆地破坏了黄曲霉的分生孢子和菌丝体形态，并由于质膜完整性降低而导致电解质泄漏。它在黄曲霉中诱导细胞凋亡以及形态异常、磷脂酰丝氨酸外化、线粒体膜电位去极化、细胞内活性氧积累和 DNA 片段化细胞。代谢组学分析表明，1-辛醇处理破坏了不饱和脂肪酸、ATP 结合盒转运蛋白、氨基酸代谢和甘油磷脂代谢的生物合成。这项研究证明了 1-辛醇作为一种生物熏蒸剂在防止收获后谷物真菌腐败方面具有广阔的应用潜力。