The accurate and sensitive detection of survivin mRNA is of great significance for cancer diagnosis and treatment. However, limited by the low-abundance mRNA in live cells, most strategies of survivin mRNA detection that were one-to-one signal-triggered model (one target triggered one signal) were inapplicable in practice. Here, we reported a binding-induced DNAzyme motor triggered by the survivin mRNA, which was a one-to-more signal-triggered model (one target triggered more signals), amplifying the detection signal and enhancing the sensitivity. The nanomotor is constructed by assembling several DNAzyme motor strands silenced by the blocker strands, and dozens of FAM-labeled substrate strands on a single gold nanoparticle (AuNP), forming three-dimensional DNA tracks. Through building the survivin mRNA bridge between the blocker and the DNAzyme motor strand, the binding-induced DNA nanomotor could be triggered by survivin mRNA. The operation of the DNAzyme motor was self-powered. And each walking step of the DNAzyme motor was fueled by DNAzyme-catalyzed substrate cleavage, along with the cleavage of the fluorescent molecule, resulting in autonomous and progressive walking along the AuNP-based tracks, and the fluorescence increase. The DNAzyme motor exhibited excellent sensitivity and remarkable specificity for survivin mRNA, providing the potential for cell image.

survivin mRNA的准确、灵敏检测对于癌症的诊断和治疗具有重要意义。然而，受限于活细胞中低丰度的mRNA，大多数采用一对一信号触发模型（一个目标触发一个信号）的survivin mRNA检测策略在实践中并不适用。在这里，我们报道了一种由 survivin mRNA 触发的结合诱导的 DNAzyme 马达，这是一个一对多的信号触发模型（一个目标触发更多信号），放大检测信号并提高灵敏度。纳米马达是通过在单个金纳米颗粒 (AuNP) 上组装几条被阻断剂链沉默的 DNAzyme 马达链和数十条 FAM 标记的底物链构成的，形成三维 DNA 轨道。通过在阻断剂和 DNAzyme 运动链之间建立 survivin mRNA 桥，结合诱导的 DNA nanomotor 可以由 survivin mRNA 触发。DNAzyme 马达的操作是自供电的。DNAzyme 马达的每个行走步骤都由 DNAzyme 催化的底物切割以及荧光分子的切割提供动力，导致沿着基于 AuNP 的轨道自主和渐进地行走，并且荧光增加。DNAzyme 马达对 survivin mRNA 表现出优异的敏感性和显着的特异性，为细胞成像提供了潜力。随着荧光分子的切割，导致沿着基于AuNP的轨道自主和渐进地行走，并且荧光增加。DNAzyme 马达对 survivin mRNA 表现出优异的敏感性和显着的特异性，为细胞成像提供了潜力。随着荧光分子的切割，导致沿着基于AuNP的轨道自主和渐进地行走，并且荧光增加。DNAzyme 马达对 survivin mRNA 表现出优异的敏感性和显着的特异性，为细胞成像提供了潜力。