ATP is the direct source of energy required for all life activities and can be used as a cancer biomarker. Although several fluorescent probes are developed to detect ATP, most of them have the problem of poor stability or short emission that is not suitable for in-vivo imaging. Herein, three ATP-responsive near-infrared (NIR) fluorescent nanoprobes are prepared by encapsulating NIR fluorescent dyes in the pores of ZIF-90. Among them, CP@ZIF-90 nanoprobe has the longest emission wavelength (705 nm) and the largest fluorescence enhancement (32-fold) for ATP detection. Moreover, the nanoprobe has excellent photostability and chemical stability. It is worth to point that carboxyl group in fluorescent dyes plays the important role, which provides a direction for the construction of NIR fluorescent nanoprobes based on ZIF-90. In addition, CP@ZIF-90 has good biocompatibility and the ability to enrich in tumor. It is successfully used for fluorescence imaging of endogenous and exogenous ATP levels in real-time in living cells (293 T and CT26 cells). More importantly, CP@ZIF-90 has shown excellent ability in distinguishing normal mice from tumor mice, which make it have the possibility of being an effective technique for cancer early diagnosis in vivo by imaging ATP.

ATP是所有生命活动所需的直接能量来源，可用作癌症生物标志物。尽管开发了几种荧光探针来检测ATP，但大多数都存在稳定性差或发射时间短的问题，不适用于体内成像。在此，通过将 NIR 荧光染料封装在 ZIF-90 的孔中，制备了三种 ATP 响应的近红外 (NIR) 荧光纳米探针。其中，CP@ZIF-90纳米探针发射波长最长（705 nm），对ATP检测的荧光增强最大（32倍）。此外，纳米探针具有优异的光稳定性和化学稳定性。值得指出的是，荧光染料中的羧基起着重要作用，这为构建基于ZIF-90的近红外荧光纳米探针提供了方向。此外，CP@ZIF-90具有良好的生物相容性和肿瘤富集能力。它成功地用于活细胞（293 T 和 CT26 细胞）中内源性和外源性 ATP 水平的实时荧光成像。更重要的是，CP@ZIF-90在区分正常小鼠和肿瘤小鼠方面表现出优异的能力，使其有可能成为通过ATP成像进行体内癌症早期诊断的有效技术。