10-Hydroxydec-2-Enoic Acid 通过拯救蛋白质和能量代谢来减少羟基自由基对血管平滑肌细胞的损伤。,Frontiers in Nutrition

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10-Hydroxydec-2-Enoic Acid 通过拯救蛋白质和能量代谢来减少羟基自由基对血管平滑肌细胞的损伤。
Frontiers in Nutrition ( IF 4.0 ) Pub Date : 2022-05-26 , DOI: 10.3389/fnut.2022.873892
Pei Fan _{1,

2} , Fangfang Sha ₂ , Chuan Ma ₁ , Qiaohong Wei ₁ , Yaqi Zhou ₂ , Jing Shi ₂ , Jiaojiao Fu ₂ , Lu Zhang ₂ , Bin Han ₁ , Jianke Li ₁

Affiliation

10-Hydroxydec-2-enoic acid (10-HDA) 是一种来自天然食品蜂王浆的不饱和羟基脂肪酸，可以保护细胞和组织免受损伤，但其潜在机制仍未探索。我们假设最活泼的氧物质羟基自由基(•OH) 的中和是10-HDA 细胞保护作用的重要因素。在本研究中，我们发现 10-HDA (2%, g/ml) 对•OH 的清除率超过 20%，这是通过多步氧化 -OH 基团和 10 的 C=C 键实现的。 -HDA。此外，10-HDA 显着增强了受 •OH 损伤的血管平滑肌细胞 (VSMC) 的活力（P < 0.01），显着减弱了代表细胞脂质过氧化的 •OH 衍生的丙二醛产生（P < 0.05），并显着增加•OH 应激的VSMC 中的谷胱甘肽水平（P < 0.05），表明10-HDA 在降低•OH 诱导的细胞毒性中的作用。对 VSMC 的进一步蛋白质组学分析确定了 195 种通过 •OH 攻击降低表达的蛋白质，这些蛋白质被 10-HDA 拯救上调，主要参与蛋白质合成（如翻译、蛋白质转运、核糖体和 RNA 结合）和能量代谢（如脂肪酸降解和糖酵解/糖异生）。总之，这些发现表明10-HDA 可以通过拮抗•OH 诱导的VSMCs 损伤来有效促进细胞存活。据我们所知，我们的结果提供了第一个具体证据，表明 10-HDA 清除的•OH 可能是维持血管健康的潜在药理学应用。

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10-Hydroxydec-2-Enoic Acid Reduces Hydroxyl Free Radical-Induced Damage to Vascular Smooth Muscle Cells by Rescuing Protein and Energy Metabolism.

10-Hydroxydec-2-enoic acid (10-HDA), an unsaturated hydroxyl fatty acid from the natural food royal jelly, can protect against cell and tissue damage, yet the underlying mechanisms are still unexplored. We hypothesized that the neutralization of the hydroxyl free radical (•OH), the most reactive oxygen species, is an important factor underlying the cytoprotective effect of 10-HDA. In this study, we found that the •OH scavenging rate by 10-HDA (2%, g/ml) was more than 20%, which was achieved through multiple-step oxidization of the -OH group and C=C bond of 10-HDA. Moreover, 10-HDA significantly enhanced the viability of vascular smooth muscle cells (VSMCs) damaged by •OH (P < 0.01), significantly attenuated •OH-derived malondialdehyde production that represents cellular lipid peroxidation (P < 0.05), and significantly increased the glutathione levels in •OH-stressed VSMCs (P < 0.05), indicating the role of 10-HDA in reducing •OH-induced cytotoxicity. Further proteomic analyses of VSMCs identified 195 proteins with decreased expression by •OH challenge that were upregulated by 10-HDA rescue and were primarily involved in protein synthesis (such as translation, protein transport, ribosome, and RNA binding) and energy metabolism (such as fatty acid degradation and glycolysis/gluconeogenesis). Taken together, these findings indicate that 10-HDA can effectively promote cell survival by antagonizing •OH-induced injury in VSMCs. To the best of our knowledge, our results provide the first concrete evidence that 10-HDA-scavenged •OH could be a potential pharmacological application for maintaining vascular health.

更新日期：2022-05-26

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