The normal operation of lysosome, mitochondria, Golgi apparatus and endoplasmic reticulum plays a significant role in maintaining cell homeostasis. Reflecting the state and function of lysosomes, viscosity is a pivotal parameter to assess the stability of microenvironment. Herein, based on TICT mechanism, a new NIR pH-dependent fluorescent probe DCIC with push-pull electronic moiety was synthesized to identify the lysosomes viscosity. In viscous media, DCIC was highly sensitive to viscosity, fluorescence intensity increased by 180 times as viscosity increased from 1.0 cp to 438.4 cp. In addition, DCIC have high localization ability for lysosome, mitochondria, Golgi apparatus, and endoplasmic reticulum and can monitor lysosomal viscosity fluctuations with laser confocal microscopy.

溶酶体、线粒体、高尔基体和内质网的正常运作对维持细胞稳态具有重要作用。粘度反映溶酶体的状态和功能，是评估微环境稳定性的关键参数。在此，基于 TICT 机制，合成了一种新的具有推挽电子部分的 NIR pH 依赖性荧光探针 DCIC，以识别溶酶体粘度。在粘性介质中，DCIC对粘度高度敏感，随着粘度从1.0 cp增加到438.4 cp，荧光强度增加了180倍。此外，DCIC对溶酶体、线粒体、高尔基体和内质网具有较高的定位能力，可通过激光共聚焦显微镜监测溶酶体粘度波动。