Orientin 通过 Nrf2/Keap1 通路抑制氧化应激减轻 d-GalN/LPS 诱导的肝损伤,Journal of Agricultural and Food Chemistry

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Orientin 通过 Nrf2/Keap1 通路抑制氧化应激减轻 d-GalN/LPS 诱导的肝损伤
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-06-21 , DOI: 10.1021/acs.jafc.2c02015
Fuhua Li _{1,

2} , Xia Liao ₁ , Ling Jiang ₁ , Jichun Zhao _{1,

2} , Surui Wu ₃ , Jian Ming _{1,

2}

Affiliation

氧化应激与肝脏疾病的发病机制有关，包括肝损伤，这是一个世界范围内的严重健康问题。天然多酚作为预防和治疗肝病的潜在药物引起了越来越多的关注。Orientin 是一种具有抗氧化能力的类黄酮成分，被认为是一种有前途的肝损伤患者的营养保健品。本研究旨在通过H ₂ O ₂诱导的HepG2氧化损伤模型研究荠菜素对d-半乳糖胺和脂多糖（d -GalN/LPS）诱导的小鼠肝损伤的改善作用，重点探讨其潜在机制。细胞。结果表明，荠菜素可减轻d-GalN/LPS通过改善肝脏组织学变化和降低肝脏和血清丙氨酸氨基转移酶和天冬氨酸氨基转移酶的水平来诱导肝损伤。此外，通过降低肝脏丙二醛、蛋白质羰基、髓过氧化物酶、一氧化氮、谷胱甘肽、谷胱甘肽过氧化物酶、谷胱甘肽还原酶和超氧化物歧化酶的水平，补充荭草素可提高小鼠的抗氧化能力。Orientin 治疗显着提高了肝脏和 HepG2 细胞中核因子红细胞 2 相关因子 2、Kelch 样 ECH 相关蛋白 1、血红素加氧酶 1 和烟酰胺醌氧化还原酶 1 的蛋白质和 mRNA 表达。荠菜素的管理还提高了 HepG2 细胞中谷胱甘肽 S-转移酶和 Maf 的蛋白质表达。综合起来，

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Orientin Attenuated d-GalN/LPS-Induced Liver Injury through the Inhibition of Oxidative Stress via Nrf2/Keap1 Pathway

Oxidative stress is involved in the pathogenesis of liver diseases, including liver injury, a serious health problem worldwide. Natural polyphenols have attracted increasing attention as potential agents for the prevention and treatment of liver diseases. Orientin, a flavonoid component with antioxidant capacity, has been regarded as a promising nutraceutical for patients with liver damage. This study aimed to investigate the amelioration effect of orientin on d-galactosamine and lipopolysaccharides (d-GalN/LPS) induced liver injury in mice, with a focus on its underlying mechanisms by using the H₂O₂-induced oxidative damage model of HepG2 cells. Results indicated that orientin alleviated d-GalN/LPS-induced liver damage by improving the hepatic histological changes and reducing the levels of hepatic and serum alanine aminotransferase and aspartic acid aminotransferase. Additionally, supplementation of orientin improved the antioxidant ability in mice by decreasing the levels of hepatic malondialdehyde, protein carbonyl, myeloperoxidase, nitric oxide, glutathione, glutathione peroxidase, gluathione reductase, and superoxide dismutase. Orientin treatment significantly elevated both the protein and mRNA expressions of nuclear factor erythroid 2-related factor 2, Kelch-like ECH-associated protein-1, heme oxygenase-1, and nicotinamide quinone oxidoreductase 1 in liver and HepG2 cells. The management of orientin also elevated the protein expression of glutathione S-transferase and Maf in HepG2 cells. Taken together, it suggested that orientin played an amelioration effect on liver injury by suppressing oxidative stress, which might be strongly related to the activation of Nrf2/ARE through PI3K/Akt and P38/MAPK signal pathways.

更新日期：2022-06-21

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