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Core and rod structures of a thermophilic cyanobacterial light-harvesting phycobilisome
Nature Communications ( IF 14.7 ) Pub Date : 2022-06-17 , DOI: 10.1038/s41467-022-30962-9
Keisuke Kawakami 1 , Tasuku Hamaguchi 1 , Yuu Hirose 2 , Daisuke Kosumi 3 , Makoto Miyata 4 , Nobuo Kamiya 5 , Koji Yonekura 1, 6, 7
Affiliation  

Cyanobacteria, glaucophytes, and rhodophytes utilize giant, light-harvesting phycobilisomes (PBSs) for capturing solar energy and conveying it to photosynthetic reaction centers. PBSs are compositionally and structurally diverse, and exceedingly complex, all of which pose a challenge for a comprehensive understanding of their function. To date, three detailed architectures of PBSs by cryo-electron microscopy (cryo-EM) have been described: a hemiellipsoidal type, a block-type from rhodophytes, and a cyanobacterial hemidiscoidal-type. Here, we report cryo-EM structures of a pentacylindrical allophycocyanin core and phycocyanin-containing rod of a thermophilic cyanobacterial hemidiscoidal PBS. The structures define the spatial arrangement of protein subunits and chromophores, crucial for deciphering the energy transfer mechanism. They reveal how the pentacylindrical core is formed, identify key interactions between linker proteins and the bilin chromophores, and indicate pathways for unidirectional energy transfer.



中文翻译:

嗜热蓝藻光捕获藻胆体的核心和杆状结构

蓝藻、灰藻和红藻利用巨大的光捕获藻胆体 (PBS) 捕获太阳能并将其输送到光合反应中心。PBS 在组成和结构上多种多样,并且极其复杂,所有这些都对全面了解其功能提出了挑战。迄今为止,已经通过低温电子显微镜 (cryo-EM) 描述了 PBS 的三种详细结构:半椭圆体型、来自红藻的块型和蓝藻半盘型。在这里,我们报告了嗜热蓝藻半盘状 PBS 的五圆柱别藻蓝蛋白核心和含藻蓝蛋白棒的冷冻电镜结构。这些结构定义了蛋白质亚基和发色团的空间排列,这对于破译能量转移机制至关重要。

更新日期:2022-06-20
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