The construction of lignin nanoparticles (LNPs) with both lignin properties and nanomaterial properties through controlling the morphologies and structures of lignin is one of the effective ways to realize its application in the field of biomedicine. Firstly, the morphology and chemical structure of LNPs were studied in detailed. The results showed that the chemical structural characteristics of LNPs had not changed significantly and its morphology was more regular shape and narrower size distribution (50–350 nm). Besides, LNPs also exhibited excellent water dispersion stability and high negative zeta potential. Subsequently, LNPs as wound dressings had good antioxidant property, excellent adsorption capacity of protein, outstanding bactericidal activity and remarkable biocompatibility, suggesting that LNPs did not interfere with cell proliferation during wound healing. Finally, the in vivo results of mouse wounds further illustrated that treatment of wounded skin wounds with LNPs enhanced its effective healing. After 15 days, as compared with the untreated control and original lignin (OL) groups, the wounds treated of LNPs was completely closed and granulation tissue formation was advanced. Overall, this study can be a good method for high-value applications of LNPs, and highlighting the advantages of using lignin as medical adjuvant nanomaterials to accelerate wound healing.

通过控制木质素的形貌和结构，构建兼具木质素特性和纳米材料特性的木质素纳米颗粒（LNPs）是实现其在生物医学领域应用的有效途径之一。首先，详细研究了LNPs的形态和化学结构。结果表明，LNPs的化学结构特征没有明显变化，其形态更规则，尺寸分布更窄（50-350 nm）。此外，LNPs 还表现出优异的水分散稳定性和较高的负 zeta 电位。随后，作为伤口敷料的LNPs具有良好的抗氧化性能、优异的蛋白质吸附能力、突出的杀菌活性和显着的生物相容性，表明 LNP 在伤口愈合过程中不会干扰细胞增殖。最后，小鼠伤口的体内结果进一步表明，用 LNPs 治疗受伤的皮肤伤口可增强其有效愈合。15天后，与未处理的对照组和原始木质素（OL）组相比，LNPs处理的伤口完全闭合，肉芽组织形成提前。总体而言，这项研究可以成为 LNPs 高价值应用的一个很好的方法，并突出了使用木质素作为医用辅助纳米材料加速伤口愈合的优势。LNPs治疗的伤口完全闭合，肉芽组织形成提前。总体而言，这项研究可以成为 LNPs 高价值应用的一个很好的方法，并突出了使用木质素作为医用辅助纳米材料加速伤口愈合的优势。LNPs治疗的伤口完全闭合，肉芽组织形成提前。总体而言，这项研究可以成为 LNPs 高价值应用的一个很好的方法，并突出了使用木质素作为医用辅助纳米材料加速伤口愈合的优势。