Exploration on the Cr(VI) resistance mechanism of a novel thermophilic Cr(VI)-reducing bacteria Anoxybacillus flavithermus ABF1 isolated from Tengchong geothermal region, China,Environmental Microbiology Reports

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Exploration on the Cr(VI) resistance mechanism of a novel thermophilic Cr(VI)-reducing bacteria Anoxybacillus flavithermus ABF1 isolated from Tengchong geothermal region, China
Environmental Microbiology Reports ( IF 3.6 ) Pub Date : 2022-06-14 , DOI: 10.1111/1758-2229.13070
Wenjing Yang ₁ , Wanqi Hong ₁ , Yongji Huang ₁ , Shuzhen Li ₁ , Mengke Li ₁ , Hui Zhong ₂ , Zhiguo He ₁

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Hexavalent chromium resistance and reduction mechanisms of microorganism provide a critical guidance for Cr(VI) bioremediation. However, related researches are limited in mesophiles and deficient for thermophiles. In this work, a novel alkaline Cr(VI)-reducing thermophile Anoxybacillus flavithermus ABF1 was isolated from geothermal region. The mechanisms of Cr(VI) resistance and reduction were investigated. The results demonstrated that A. flavithermus ABF1 could survive in a wide temperature range from 50°C to 70°C and in pH range of 7.0–9.0. Strain ABF1 showed excellent growth activity and Cr(VI) removal performance when initial Cr(VI) concentration was lower than 200 mg L⁻¹. 93.71% of Cr(VI) was removed at initial concentration of 20 mg L⁻¹ after 72 h. The majority of Cr(VI) was found to be reduced extracellularly by enzymes secreted by cells. XPS and Raman analysis further manifested that Cr₂O₃ was the product of Cr(VI) reduction. Moreover, the Cr(VI) transportation-related gene cysP and Cr(VI) reduction-related gene azoR of A. flavithermus ABF1 played key roles in inhibiting Cr(VI) entering cells and promoting extracellular Cr(VI) reduction respectively. This work provides novel insight into the mechanisms of Cr(VI) resistance and detoxication of thermophiles, which leads to a promising alternative strategy for heavy metal bioremediation in areas with elevated temperature.

中文翻译：

腾冲地热区一株新型嗜热Cr(VI)还原菌Anoxybacillus flavithermus ABF1抗Cr(VI)机制探索

微生物的六价铬抗性和还原机制为Cr(VI)生物修复提供了重要指导。然而，相关研究仅限于嗜温菌，缺乏嗜热菌。在这项工作中，从地热区分离出一种新型的碱性 Cr(VI) 还原嗜热菌Anoxybacillus flavithermus ABF1。研究了Cr(VI)的抗性和还原机制。结果表明，A . flavithermus ABF1可以在 50°C 至 70°C 的宽温度范围和 7.0–9.0 的 pH 范围内存活。当初始 Cr(VI) 浓度低于 200 mg L ^{-1时，菌株 ABF1 表现出优异的生长活性和 Cr(VI) 去除性能}. 72 小时后，初始浓度为 20 mg L ^{-1时，93.71% 的 Cr(VI) 被去除。}发现大部分 Cr(VI) 在细胞外被细胞分泌的酶还原。XPS和拉曼分析进一步表明Cr ₂ O ₃是Cr(VI)还原的产物。此外， A的Cr(VI) 运输相关基因cysP和 Cr(VI) 还原相关基因azoR。黄曲霉ABF1分别在抑制Cr(VI)进入细胞和促进细胞外Cr(VI)还原中起关键作用。这项工作提供了对 Cr(VI) 抗性和嗜热菌解毒机制的新见解，从而为高温地区的重金属生物修复提供了一种有前景的替代策略。

更新日期：2022-06-14

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