Accumulation of plastic materials in terrestrial systems threatens to contaminate food chains. The aim of the current study is to determine the impact of microplastics synthesized from PET plastics (control, 50, 250, 500, 750, 1000 mg/L) with respect to morphological, biochemical impact on Cicer arietinum using standardized 72 h assay and cytotoxicity study on Allium cepa root tips. The synthesized microplastics were characterized by Scanning Electron Microscope (SEM) and Fourier Transform Infrared spectroscopy (FTIR) studies. Germination studies clearly revealed that there is a sharp decrease in germination with increasing the concentration of microplastics. Both pigment and carbohydrate levels increased up to 500 mg/L concentration, although protein levels increased with increase of microplastic dose. Catalase activity also increased with increasing microplastic concentration. Finally, cytotoxicity studies revealed significant chromosomal aberration at higher dose of microplastics. Therefore, it may be concluded that the microplastics have significant biological and structural adverse effects on plant metabolism.