Naringenin, a flavanone, has been reported for a wide range of pharmacological activities. However, there are few reports on the absorption, transport and antioxidant effects of naringenin. The study was to explore the uptake, transport and antioxidant effects of naringenin in vitro. Cell transmembrane resistance, lucifer yellow transmission rate, and alkaline phosphatase activity were used to evaluate the successful construction of cell model. The results showed that the absorption and transport of naringenin by Caco-2 cells were time- and concentration-dependent. Different temperatures (37 and 4°C) had a significant effect on the uptake and transport of naringenin. Verapamil, potent inhibitor of P-glycoprotein, significantly inhibit naringenin transport in Caco-2 cells. The results revealed that naringenin was a moderately absorbed biological macromolecule and can penetrate Caco-2 cells, mainly mediated by the active transport pathway involved in P-glycoprotein. At the same time, naringenin pretreatment could significantly increase the viability of H2O2-induced Caco-2 cells. Twenty four differential metabolites were identified based on cellular metabolite analysis, mainly including alanine, aspartate and glutamate metabolism, histidine metabolism, taurine and hypotaurine metabolism, pyruvate metabolism, purine metabolism, arginine biosynthesis, citrate cycle, riboflavin metabolism, and D-glutamine and D-glutamate metabolism. We concluded that the transport of naringenin by Caco-2 cells is mainly involved in active transport mediated by P-glycoprotein and naringenin may play an important role in oxidative stress-induced intestinal diseases.

中文翻译：

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柚皮素的摄取和转运及其在人肠上皮 Caco-2 细胞中的抗氧化作用。

据报道，柚皮素是一种黄烷酮，具有广泛的药理活性。然而，关于柚皮素的吸收、转运和抗氧化作用的报道很少。该研究旨在探索柚皮素在体外的摄取、转运和抗氧化作用。采用细胞跨膜阻力、荧光黄透过率、碱性磷酸酶活性等指标评价细胞模型的成功构建。结果表明，Caco-2细胞对柚皮素的吸收和转运具有时间和浓度依赖性。不同温度（37 和 4°C）对柚皮素的吸收和转运有显着影响。Verapamil 是 P-糖蛋白的有效抑制剂，可显着抑制 Caco-2 细胞中柚皮素的转运。结果表明，柚皮素是一种中等吸收的生物大分子，可以穿透Caco-2细胞，主要由参与P-糖蛋白的主动转运途径介导。同时，柚皮素预处理可以显着提高H2O2诱导的Caco-2细胞的活力。基于细胞代谢物分析鉴定出24种差异代谢物，主要包括丙氨酸、天冬氨酸和谷氨酸代谢、组氨酸代谢、牛磺酸和亚牛磺酸代谢、丙酮酸代谢、嘌呤代谢、精氨酸生物合成、柠檬酸循环、核黄素代谢以及D-谷氨酰胺和D -谷氨酸代谢。