A surge of nanozymes with oxidase-like activities is emerging in various fields, whereas nanozymes with the ability to catalyze the oxidation of saccharides have less been explored. Herein, CuO nanoparticles (NPs) with phosphate-supported fructose oxidase-like activity have been reported. Notably, reactive oxygen species (ROS) have been confirmed as the products during the process. By coupling the fructose oxidase-like activity with the peroxidase-like activity of CuO NPs, a tandem catalysis-based fructose sensor can be fabricated. In detail, CuO NPs can catalyze the fructose oxidation under O₂ to yield ROS (e.g., H₂O₂, •OH, and O₂^·-) and effectively decompose H₂O₂ into ·OH. After that, terephthalic acid can be oxidized by •OH produced from the tandem catalysis to generate a fluorescent product. This sensor shows a linear range toward fructose (0.625–275 μМ) with a low limit of detection (0.5 μМ), which can be successfully conducted to detect fructose from real samples. Overall, this work aims to expand the catalytic types of nanozymes and provide a desirable fructose sensor.

大量具有类氧化酶活性的纳米酶正在各个领域涌现，而具有催化糖类氧化能力的纳米酶的探索较少。在此，已经报道了具有磷酸盐支持的果糖氧化酶样活性的 CuO 纳米颗粒 (NPs)。值得注意的是，活性氧（ROS）已被确认为该过程中的产物。通过将果糖氧化酶样活性与 CuO NPs 的过氧化物酶样活性相结合，可以制造基于串联催化的果糖传感器。具体来说，CuO NPs可以在O ₂下催化果糖氧化产生ROS（如H ₂ O ₂、•OH和O ₂ ^{· -}）并有效分解H ₂ O ₂成· OH。之后，对苯二甲酸可以被串联催化产生的•OH氧化，生成荧光产物。该传感器显示果糖的线性范围 (0.625–275 μМ)，检测限低 (0.5 μМ)，可成功用于检测实际样品中的果糖。总体而言，这项工作旨在扩展纳米酶的催化类型并提供理想的果糖传感器。