Thermo-responsive Raman-enhanced nanocapsules were successfully fabricated by Pluronic® F127 (F127) decorated with gold nanoparticles (AuNPs) for surface-enhanced Raman scattering (SERS) detection of biomolecules. F127 nanocapsules changes from hydrophilicity (swelling) to hydrophobicity (de-swelling) when the temperature increases from 15 °C to 37 °C, owing to the lower critical solution temperature (LCST) of F127 is about 26.5 °C. The size of nanocapsules would be enormous shrinking from 160 nm to 20 nm, resulting in a significant decrease in the distance between AuNPs to enhance hot spot effect, which increases the sensitivity of SERS detection. Based on the thermo-sensitive behavior, the ratio of AuNPs and F127 would be manipulated to find the optimal SERS enhancement effect. SERS nanocapsules can rapidly detect biomolecules (adenine and R6G) with limit of detection (LOD) lower than 10⁻⁶ M. In addition, the relatively difficult to detect clinical samples, carboxyl-terminal parathyroid hormone fragments (C-PTH), can also be measured by the thermo-responsive SERS nanocapsules developed in this work. It is expected the biomolecules can be adsorbed at low temperature (15 °C), as well as collected and concentrated at high temperature (37 °C) for SERS detection, to increase the sensitivity and stability of SERS detection.

用金纳米粒子 (AuNPs) 修饰的 Pluronic® F127 (F127) 成功制造了热响应拉曼增强纳米胶囊，用于生物分子的表面增强拉曼散射 (SERS) 检测。当温度从 15 °C 升高到 37 °C 时，F127 纳米胶囊从亲水性（溶胀）变为疏水性（去溶胀），这是由于 F127 的较低临界溶解温度（LCST）约为 26.5 °C。纳米胶囊的尺寸将从 160 nm 大幅缩小到 20 nm，导致 AuNPs 之间的距离显着减小以增强热点效应，从而提高 SERS 检测的灵敏度。基于热敏行为，将操纵AuNPs和F127的比例以找到最佳的SERS增强效果。^-6  M。此外，相对难以检测的临床样品，羧基末端甲状旁腺激素片段（C-PTH），也可以通过本工作开发的热响应SERS纳米胶囊进行测量。预计生物分子可以在低温（15°C）下吸附，并在高温（37°C）下收集和浓缩用于SERS检测，以提高SERS检测的灵敏度和稳定性。