Molecular Mechanism of Sclerotinia sclerotiorum Resistance to Succinate Dehydrogenase Inhibitor Fungicides,Journal of Agricultural and Food Chemistry

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Molecular Mechanism of Sclerotinia sclerotiorum Resistance to Succinate Dehydrogenase Inhibitor Fungicides
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-06-06 , DOI: 10.1021/acs.jafc.2c02056
Qiao Wang ₁ , Yushuai Mao ₁ , Shengxue Li ₁ , Tao Li ₁ , Jianxin Wang _{1,

2} , Mingguo Zhou _{1,

2} , Yabing Duan _{1,

2}

Affiliation

Succinate dehydrogenase inhibitor (SDHI) fungicides have a wide spectrum of fungicidal effects on a variety of fungi causing plant diseases, including Sclerotinia stem rot caused by Sclerotinia sclerotiorum. However, the consistent use of site-specific SDHI fungicides can result in the development of resistant isolates with mutations in the SDHB, SDHC, or SDHD subunit thereby leading to a rapid decline of fungicide performance. In this study, we found that SDHC was genetically evolved into two isotypes SDHC1 and SDHC2 in S. sclerotiorum but not involved in the sensitivity to SDHI fungicides. In addition, we demonstrated that the A11V substitution in SDHB was not involved in the resistance of S. sclerotiorum to boscalid, and this substitution widely emerged in the field populations. Meanwhile, the P226L substitution in SDHB was demonstrated to confer boscalid resistance in S. sclerotiorum. The result of cross-resistance showed that the SDHB-P226L substitution exhibited a positive cross-resistance between boscalid and carboxin, fluopyram, pydiflumetofen, flubeneteram, pyraziflumid, fluindapyr, or penthiopyrad. Taken together, our results indicated that the P226L substitution in SDHB resulted in the resistance of S. sclerotiorum to SDHI fungicides but suffered from fitness penalty, especially the homozygous mutants conferring the P226L substitution in SDHB.

中文翻译：

核盘菌对琥珀酸脱氢酶抑制剂杀菌剂抗性的分子机制

琥珀酸脱氢酶抑制剂（SDHI）杀菌剂对多种引起植物病害的真菌具有广谱杀菌作用，包括由核盘菌引起的核盘菌茎腐病。然而，持续使用位点特异性 SDHI 杀菌剂会导致产生具有 SDHB、SDHC 或 SDHD 亚基突变的耐药菌株，从而导致杀菌剂性能迅速下降。在本研究中，我们发现SDHC 在核盘菌中遗传进化为SDHC1 和SDHC2 两种同种型，但不参与对SDHI 杀菌剂的敏感性。此外，我们证明了 SDHB 中的 A11V 替代与核盘菌的抗性无关到啶酰菌胺，这种替代在田间种群中广泛出现。同时，SDHB 中的 P226L 替代被证明在S. sclerotiorum中赋予了啶酰菌胺抗性。交叉耐药性结果表明，SDHB-P226L替代品在啶酰菌胺与羧苄青霉素、氟吡菌酰胺、吡氟美托芬、氟苯醚菌胺、吡唑醚菌胺、氟啶虫胺或吡噻菌胺之间表现出阳性交叉耐药性。总之，我们的结果表明，SDHB 中的 P226L 取代导致核盘菌对 SDHI 杀菌剂的抗性，但遭受适应性损失，特别是在 SDHB 中赋予 P226L 取代的纯合突变体。

更新日期：2022-06-06

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