PatDREB Transcription Factor Activates Patchoulol Synthase Gene Promoter and Positively Regulates Jasmonate-Induced Patchoulol Biosynthesis,Journal of Agricultural and Food Chemistry

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PatDREB Transcription Factor Activates Patchoulol Synthase Gene Promoter and Positively Regulates Jasmonate-Induced Patchoulol Biosynthesis
Journal of Agricultural and Food Chemistry ( IF 5.7 ) Pub Date : 2022-06-02 , DOI: 10.1021/acs.jafc.2c01660
Xiuzhen Chen _{1,

2} , Xiaobing Wang ₁ , Daidi Wu ₁ , Junren Li ₁ , Huiling Huang ₁ , Xilin Wang ₁ , Ruoting Zhan _{1,

3} , Likai Chen _{1,

3}

Affiliation

The production of patchoulol in the patchouli (Pogostemon cablin) plant determines its application value, as it is the principal active sesquiterpene of essential oil extracted from this plant. Here, the promoter of patchoulol synthase gene (PatPTS_pro) was isolated and found to be methyl jasmonate (MeJA)-induced. A nucleus-localized AP2/ERF transcription factor PatDREB was identified as a transcription activator binding to PatPTS_pro, regulating patchoulol biosynthesis through modulating the gene expression. PatDREB also interacts with jasmonate ZIM-domain 4 (JAZ4). Furthermore, PatDREB could physically interact with the MYB-related transcription factor PatSWC4 and synergistically facilitate patchoulol biosynthesis. However, the transcriptional activation activity of the PatDREB-PatSWC4 complex could be inhibited by PatJAZ4, and JA could reverse this interference. Overall, we demonstrated the positive roles of PatDREB and the PatDREB-PatSWC4 complex in regulating patchoulol production, which advance our understanding of the regulatory network of patchoulol biosynthesis.

中文翻译：

PatDREB 转录因子激活广藿香醇合酶基因启动子并正向调节茉莉酸诱导的广藿香酚生物合成

广藿香 ( Pogostemon cablin ) 植物中广藿香酚的产生决定了其应用价值，因为它是从该植物中提取的精油的主要活性倍半萜。在这里，广藿香醇合酶基因 ( PatPTS _pro ) 的启动子被分离出来，发现是茉莉酸甲酯 (MeJA) 诱导的。核定位的 AP2/ERF 转录因子 PatDREB 被鉴定为与PatPTS _{pro结合的转录激活因子}，通过调节基因表达来调节广藿香的生物合成。PatDREB 还与茉莉酸 ZIM 域 4 (JAZ4) 相互作用。此外，PatDREB 可以与 MYB 相关转录因子 PatSWC4 发生物理相互作用，并协同促进广藿香酚的生物合成。然而，PatJAZ4 可以抑制 PatDREB-PatSWC4 复合物的转录激活活性，而 JA 可以逆转这种干扰。总体而言，我们展示了 PatDREB 和 PatDREB-PatSWC4 复合物在调节广藿香醇生产中的积极作用，这促进了我们对广藿香酚生物合成调控网络的理解。

更新日期：2022-06-02

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