Analytical and Bioanalytical Chemistry ( IF 3.8 ) Pub Date : 2022-06-03 , DOI: 10.1007/s00216-022-04149-6 Xing Yuxin 1 , Sun Laipeng 1 , Liu Kang 1 , Shi Haipeng 1 , Wang Zonghua 1 , Wang Wenjing 1
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Carbon dots (CDs) have several superior characteristics including sufficient carbon sources, easy preparation, no toxicity, and high catalytic efficiency as a new kind of nanozyme. Herein, Ce-doped carbon dots (Ce-CDs), Cr-doped carbon dots (Cr-CDs), Cu-doped carbon dots (Cu-CDs), Fe-doped carbon dots (Fe-CDs), Mn-doped carbon dots (Mn-CDs), and non-metal-doped carbon dots (0-CDs) were synthesized to explore the detection of hydrogen peroxide (H2O2) and glucose as peroxidase mimic. The prepared CDs could efficiently oxidize the colorless 3,3′,5,5′-tetramethylbenzidine (TMB) into blue oxTMB in the presence of H2O2. After adding glucose oxidase (GOD) to the CDs/TMB system, a colorimetric method for glucose detection was developed. The results show that Fe-CDs possess the highest catalytic activity. When using Fe-CDs as peroxidase mimetics, the detection limit of this assay for glucose was 0.029 mmol L−1. This successfully provides a sensitive and selective colorimetric method for hydrogen peroxide and glucose determination.
Graphical abstract
中文翻译：
金属掺杂碳点作为过氧化物酶模拟物用于过氧化氢和葡萄糖检测
作为一种新型纳米酶，碳点（CDs）具有碳源充足、制备简单、无毒、催化效率高等优点。在此，Ce掺杂碳点（Ce-CDs）、Cr掺杂碳点（Cr-CDs）、Cu掺杂碳点（Cu-CDs）、Fe掺杂碳点（Fe-CDs）、Mn掺杂碳合成点 (Mn-CDs) 和非金属掺杂碳点 (0-CDs) 以探索过氧化氢 (H 2 O 2 ) 和葡萄糖作为过氧化物酶模拟物的检测。制备的CDs可以在H 2 O 2存在下有效地将无色的3,3',5,5'-四甲基联苯胺(TMB)氧化成蓝色oxTMB。. 将葡萄糖氧化酶 (GOD) 添加到 CDs/TMB 系统后，开发了一种用于葡萄糖检测的比色法。结果表明，Fe-CDs具有最高的催化活性。当使用 Fe-CDs 作为过氧化物酶模拟物时，该测定法对葡萄糖的检测限为 0.029 mmol L -1。这成功地为过氧化氢和葡萄糖的测定提供了一种灵敏且选择性的比色方法。
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