Deoxynivalenol (DON) is highly toxic to the environment and human health. It is important to detect DON with ultra-high sensitivity, ease of operation, and low cost. Inspired by the excellent stability and biocompatibility of polydopamine, a universal, portable and ultra-sensitive pipet immunoassay platform was reported for DON detection based on dopamine self-polymerization (polydopamine coating and polydopamine nanoparticles). The polydopamine coating acted as an effective strategy for biomolecule immobilization on the pipet to improve the coating efficiency that significantly reduced the required concentration of biomolecules. Performing the ELISA in pipets saved nearly 67% of the antigen amount and 83% of the antibody amount, which reduced the detection cost and simplified the experimental steps. The dual signal amplification in this pipet immunoassay enabled ultra-high sensitivity. Polydopamine nanoparticles acted as the enrichment carrier of horseradish peroxidase-goat anti-mouse IgG for the first-round signal amplification, followed by the tyramine-mediated loading of streptavidin-HRP for the second-round signal amplification. The dual-enriched HRP catalyzed the color-developing substrate to achieve highly sensitive colorimetric DON detection with a limit of detection of 0.435 ng/mL.

脱氧雪腐镰刀菌烯醇 (DON) 对环境和人类健康具有剧毒。以超高灵敏度、易于操作和低成本检测 DON 非常重要。受聚多巴胺优异的稳定性和生物相容性的启发，报道了一种基于多巴胺自聚合（聚多巴胺涂层和聚多巴胺纳米颗粒）的通用、便携式和超灵敏移液管免疫测定平台，用于检测 DON。聚多巴胺涂层作为生物分子固定在移液管上的有效策略，可提高涂层效率，显着降低所需的生物分子浓度。在移液器中进行ELISA节省了近67%的抗原量和83%的抗体量，降低了检测成本，简化了实验步骤。这种移液器免疫分析中的双重信号放大实现了超高灵敏度。聚多巴胺纳米粒作为辣根过氧化物酶-山羊抗小鼠IgG的富集载体进行第一轮信号放大，随后酪胺介导的链霉亲和素-HRP负载进行第二轮信号放大。双富集HRP催化显色底物实现高灵敏度比色DON检测，检测限为0.435 纳克/毫升。