Viscosity is an important microenvironmental indicator that plays an important role in the process of information transmission in various regions. Herein, two coumarin-based viscosity-sensitive fluorescent probes (CHB, CHN) were synthesized and the photophysical properties of the two probes were studied. The fluorescence quantum yields of CHB and CHN in glycerol can be as high as 25.2% and 18.3% respectively. The two probes can linearly detect viscosity in the viscosity logarithm range of 0.83–2.07, which is not interfered with pH, metal ions, anions and biomolecules. Fluorescent confocal cell experiments show CHB and CHN have good targeting ability to mitochondrion, lysosome, Endoplasmic reticulum and Golgi apparatus, and can be used to detect viscosity in mitochondrion/lysosome.

粘度是重要的微环境指标，在各个区域的信息传递过程中发挥着重要作用。在此，合成了两种基于香豆素的粘度敏感荧光探针（CHB，CHN），并研究了两种探针的光物理性质。CHB和CHN在甘油中的荧光量子产率分别高达25.2%和18.3%。两种探头可在0.83-2.07的粘度对数范围内线性检测粘度，不受pH、金属离子、阴离子和生物分子的干扰。荧光共聚焦细胞实验表明CHB和CHN对线粒体、溶酶体、内质网和高尔基体具有良好的靶向能力，可用于检测线粒体/溶酶体的粘度。