Transcriptome analysis reveals putative pathogenesis genes in Alternaria panax during infecting Panax notoginseng leaves,Genes & Genomics

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Transcriptome analysis reveals putative pathogenesis genes in Alternaria panax during infecting Panax notoginseng leaves
Genes & Genomics ( IF 1.6 ) Pub Date : 2022-05-27 , DOI: 10.1007/s13258-022-01241-0
Taif Shah _{1,

2} , Diqiu Liu _{1,

2} , XiuMing Cui _{1,

2}

Affiliation

Background

Alternaria panax is the causative agent of black spot disease in Panax notoginseng, which causes significant yield loss. However, the molecular mechanisms underlying its pathogenicity remain mostly unknown.

Objective

We sequenced the transcriptome of A. panax during infecting P. notoginseng leaves using next-generation RNA-seq to understand the molecular aspects of black spot disease.

Methods

In this study, we sequenced the A. panax transcriptome during infecting P. notoginseng leaves through next-generation sequencing to explore the pathogenesis genes that may be responsible for black spot disease on P. notoginseng.

Result

The de novo transcriptome assembly of A. panax produced 23,036 unigenes, of which 18,096 genes were functionally annotated by at least one protein database. GO enrichment analysis and KEGG pathways of differentially up-regulated genes suggest that most genes are associated with metabolic processes, catalytic activity, starch, and sucrose metabolism during infection. Many pathogenesis-associated genes, including genes encoding secreted proteins, candidate secreted effectors, cell wall degrading enzymes, transcription factors, and transporters, were up-regulated in A. panax during infection. In addition, the secondary metabolite biosynthesis genes, including cytochrome P450, and nonribosomal peptide synthetases, were also identified in this study.

Conclusions

Differential gene expression analysis has confirmed that A. panax infection was mainly present in the middle and final stages. The findings show that these pathogenesis-associated genes in A. panax may be critical for the P. notoginseng black spots disease.

中文翻译：

转录组分析揭示了三七叶侵染过程中三七链格孢中假定的发病基因

背景

三七黑斑病病原菌是三七黑斑病的病原菌，引起严重减产。然而，其致病性的分子机制仍然大多未知。

客观的

我们使用下一代 RNA-seq在感染三七叶期间对人参的转录组进行了测序，以了解黑斑病的分子方面。

方法

在本研究中，我们通过二代测序对感染三七叶片过程中的人参转录组进行测序，以探索可能导致三七黑斑病的发病基因。

结果

A. panax的从头转录组组装产生了 23,036 个 unigenes，其中 18,096 个基因由至少一个蛋白质数据库进行了功能注释。GO富集分析和差异上调基因的KEGG通路表明，大多数基因与感染过程中的代谢过程、催化活性、淀粉和蔗糖代谢有关。许多发病机制相关基因，包括编码分泌蛋白、候选分泌效应子、细胞壁降解酶、转录因子和转运蛋白的基因，在感染期间在人参中上调。此外，本研究还鉴定了次级代谢物生物合成基因，包括细胞色素 P450 和非核糖体肽合成酶。