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Time dynamics of stress legacy in clonal transgenerational effects: A case study on Trifolium repens
Ecology and Evolution ( IF 2.3 ) Pub Date : 2022-05-24 , DOI: 10.1002/ece3.8959
Jiaxin Quan 1, 2 , Zuzana Münzbergová 2, 3 , Vít Latzel 2
Ecology and Evolution ( IF 2.3 ) Pub Date : 2022-05-24 , DOI: 10.1002/ece3.8959
Jiaxin Quan 1, 2 , Zuzana Münzbergová 2, 3 , Vít Latzel 2
Affiliation
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Stress can be remembered by plants in a form of stress legacy that can alter future phenotypes of previously stressed plants and even phenotypes of their offspring. DNA methylation belongs among the mechanisms mediating the stress legacy. It is however not known for how long the stress legacy is carried by plants. If the legacy is long-lasting, it can become maladaptive in situations when parental–offspring environment do not match. We investigated for how long after the last exposure of a parental plant to drought can the phenotype of its clonal offspring be altered. We grew parental plants of three genotypes of Trifolium repens for five months either in control conditions or in control conditions that were interrupted with intense drought periods applied for two months in four different time slots. We also treated half of the parental plants with a demethylating agent (5-azacytidine, 5-azaC) to test for the potential role of DNA methylation in the stress memory. Then, we transplanted parental cuttings (ramets) individually to control environment and allowed them to produce offspring ramets for two months. The drought stress experienced by parents affected phenotypes of offspring ramets. The stress legacy resulted in enhanced number of offspring ramets originating from plants that experienced drought stress even 56 days before their transplantation to the control environment. 5-azaC altered transgenerational effects on offspring ramets. We confirmed that drought stress can trigger transgenerational effects in T. repens that is very likely mediated by DNA methylation. Most importantly, the stress legacy in parental plants persisted for at least 8 weeks suggesting that the stress legacy can persist in a clonal plant Trifolium repens for relatively long period. We suggest that the stress legacy should be considered in future ecological studies on clonal plants.
中文翻译:
无性系跨代效应中压力遗留的时间动态:以白三叶为例
植物可以以一种压力遗产的形式记住压力,这种压力遗产可以改变先前受胁迫植物的未来表型,甚至是其后代的表型。DNA甲基化属于介导压力遗留的机制。然而,尚不清楚植物会承受多长时间的压力。如果遗产是持久的,它可能会在父母-后代环境不匹配的情况下变得不适应。我们调查了亲本植物最后一次暴露于干旱后多长时间,其克隆后代的表型可以改变。我们种植了三种白车轴草基因型的亲本植物在控制条件下或在被强烈干旱期中断的控制条件下持续五个月,在四个不同的时间段内应用两个月。我们还用去甲基化剂(5-氮杂胞苷,5-azaC)处理了一半的亲本植物,以测试 DNA 甲基化在应激记忆中的潜在作用。然后,我们分别移植亲本插条(分株)以控制环境,并让它们产生后代分株两个月。父母经历的干旱胁迫影响了后代分株的表型。压力遗留导致来自植物的后代分株数量增加,这些植物甚至在移植到对照环境前 56 天就经历了干旱胁迫。5-azaC 改变了对后代分株的跨代效应。吨。很可能是由 DNA 甲基化介导的。最重要的是,亲本植物中的应激遗传持续至少8周,这表明应激遗传可以在克隆植物白三叶中持续相对较长的时间。我们建议在未来对克隆植物的生态研究中应考虑压力遗留问题。
更新日期:2022-05-26
中文翻译:
![](https://scdn.x-mol.com/jcss/images/paperTranslation.png)
无性系跨代效应中压力遗留的时间动态:以白三叶为例
植物可以以一种压力遗产的形式记住压力,这种压力遗产可以改变先前受胁迫植物的未来表型,甚至是其后代的表型。DNA甲基化属于介导压力遗留的机制。然而,尚不清楚植物会承受多长时间的压力。如果遗产是持久的,它可能会在父母-后代环境不匹配的情况下变得不适应。我们调查了亲本植物最后一次暴露于干旱后多长时间,其克隆后代的表型可以改变。我们种植了三种白车轴草基因型的亲本植物在控制条件下或在被强烈干旱期中断的控制条件下持续五个月,在四个不同的时间段内应用两个月。我们还用去甲基化剂(5-氮杂胞苷,5-azaC)处理了一半的亲本植物,以测试 DNA 甲基化在应激记忆中的潜在作用。然后,我们分别移植亲本插条(分株)以控制环境,并让它们产生后代分株两个月。父母经历的干旱胁迫影响了后代分株的表型。压力遗留导致来自植物的后代分株数量增加,这些植物甚至在移植到对照环境前 56 天就经历了干旱胁迫。5-azaC 改变了对后代分株的跨代效应。吨。很可能是由 DNA 甲基化介导的。最重要的是,亲本植物中的应激遗传持续至少8周,这表明应激遗传可以在克隆植物白三叶中持续相对较长的时间。我们建议在未来对克隆植物的生态研究中应考虑压力遗留问题。