The comprehensive understanding of the mechanism between gas messenger molecules and cell membrane microenvironment is of great significance for basic biology research. Sulfur dioxide (SO₂), as a vital gas molecule, is of great value in the permeability and fluidity of cell membranes. However, monitoring of sulfur dioxide in cell membranes with high temporal and spatial resolution remains a challenge. Herein, we designed and synthesized a ratiometric probe CMCB based on coumarin and indole sulfonate platform. The unsaturated CC is selected as the reaction site of SO₂, utilizing sulfonic acid groups to increase CMCB hydrophilicity to achieve efficient targeting of cell membranes. The probe CMCB features a good cells membranes targeting and a NIR emission (720 nm). Interestingly, the probe showed low detection limit, high selectivity and specificity of targeting cell membranes. We expect that this probe will serve as an ideal tool in studying gas messenger molecule on cytomembrane in vivo.

全面了解气体信使分子与细胞膜微环境的作用机制对于基础生物学研究具有重要意义。二氧化硫（SO ₂）作为一种重要的气体分子，对细胞膜的渗透性和流动性具有重要价值。然而，以高时间和空间分辨率监测细胞膜中的二氧化硫仍然是一个挑战。在此，我们设计并合成了基于香豆素和吲哚磺酸盐平台的比例探针CMCB 。选择不饱和C C 作为SO ₂的反应位点，利用磺酸基团增加CMCB的亲水性，实现对细胞膜的高效靶向。探头CMCB具有良好的细胞膜靶向性和 NIR 发射（720 nm）。有趣的是，该探针显示出低检测限、高选择性和靶向细胞膜的特异性。我们预计该探针将成为研究体内细胞膜上的气体信使分子的理想工具。