Single-atom nanozymes (SAZs) with peroxidase (POD)-like activity have good nanocatalytic tumor therapy (NCT) capabilities. However, insufficient hydrogen peroxide (H₂O₂) and hydrogen ions in the cells limit their therapeutic effects. Herein, to overcome these limitations, a biomimetic single-atom nanozyme system was developed for self-enhanced NCT. We used a previously described approach to produce platelet membrane vesicles. Using a high-temperature carbonization approach, copper SAZs with excellent POD-like activity were successfully synthesized. Finally, through physical extrusion, a proton pump inhibitor (PPI; pantoprazole sodium) and the SAZs were combined with platelet membrane vesicles to create PPS. Both in vivo and in vitro, PPS displayed good tumor-targeting and accumulation abilities. PPIs were able to simultaneously regulate the hydrogen ion, glutathione (GSH), and H₂O₂ content in tumor cells, significantly improve the catalytic ability of SAZs, and achieve self-enhanced NCT. Our in vivo studies showed that PPS had a tumor suppression rate of > 90%. PPS also limited the synthesis of GSH in cells at the source; thus, glutamine metabolism therapy and NCT were integrated into an innovative method, which provides a novel strategy for multimodal tumor therapy.

具有类似过氧化物酶 (POD) 活性的单原子纳米酶 (SAZ) 具有良好的纳米催化肿瘤治疗 (NCT) 能力。然而，细胞中的过氧化氢（H ₂ O ₂）和氢离子不足限制了它们的治疗效果。在此，为了克服这些限制，开发了一种用于自增强 NCT 的仿生单原子纳米酶系统。我们使用先前描述的方法来生产血小板膜囊泡。采用高温碳化方法，成功合成了具有优异类 POD 活性的铜 SAZ。最后，通过物理挤压，质子泵抑制剂（PPI；泮托拉唑钠）和 SAZ 与血小板膜囊泡结合形成 PPS。体内和体外_, PPS displayed good tumor-targeting and accumulation abilities. PPIs were able to simultaneously regulate the hydrogen ion, glutathione (GSH), and H₂O₂ content in tumor cells, significantly improve the catalytic ability of SAZs, and achieve self-enhanced NCT. Our in vivo studies showed that PPS had a tumor suppression rate of > 90%. PPS also limited the synthesis of GSH in cells at the source; thus, glutamine metabolism therapy and NCT were integrated into an innovative method, which provides a novel strategy for multimodal tumor therapy.