Astaxanthin and phycocyanin were recommended for their bioactivities. However, limited stability and low bioavailability were not conducive to their applications in the food industry. A pH-sensitive double W₁/O/W₂ emulsion-filled hydrogel loaded concomitantly with astaxanthin and phycocyanin was prepared using the two-step emulsification process. First, the W₁/O emulsion was fabricated using polyglycerol polyricinoleate as a hydrophobic emulsifier. Second, W₁/O/W₂ emulsions are typically formed by homogenizing W₁/O emulsions with an aqueous phase containing sodium caseinate. Later, gellan gum was added to the double emulsion as an additional stabilizer and gelling agent to prepare the double emulsion-filled hydrogel. The influence of gellan gum on the microstructure, rheology, and stability of double emulsions was investigated. The double emulsions remained stable during 30 days’ storage when the content of gellan gum was above 0.4% since the hydrogel formation. The microstructure of double emulsions was maintained under the acidic environment due to the network formed by gellan gum while transformed from hydrogel state to solution state since the disintegration of gellan gum network under neutral conditions. Further, based on the pH-sensitive nature of double emulsion-filled hydrogel, the co-encapsulation of phycocyanin and astaxanthin and the in vitro release behavior were demonstrated. The maximum encapsulation efficiency of phycocyanin and astaxanthin was 94.1% and 90.82%, respectively. In vitro digestion proved their intestinal targeted release ability. These results suggested that the double emulsion-filled gellan gum hydrogel can be used as a pH-sensitive carrier for intestinal targeted delivery of hydrophobic and hydrophilic food bioactive ingredients.

虾青素和藻蓝蛋白因其生物活性而被推荐。然而，有限的稳定性和低生物利用度不利于它们在食品工业中的应用。采用两步乳化法制备了同时负载虾青素和藻蓝蛋白的pH敏感双W ₁ /O/W _{2乳液填充水凝胶。}首先，使用聚甘油聚蓖麻油酸酯作为疏水乳化剂制备W _{1 /O 乳液。}其次，W ₁ /O/W ₂乳液通常通过均质化 W _1形成/O乳液与含有酪蛋白酸钠的水相。随后，将结冷胶添加到双乳液中作为额外的稳定剂和胶凝剂，以制备双乳液填充的水凝胶。研究了结冷胶对双乳液的微观结构、流变性和稳定性的影响。自水凝胶形成以来，当结冷胶的含量高于0.4%时，双乳液在30天的储存期间保持稳定。双乳液的微观结构在酸性环境下由于结冷胶形成的网络而得以维持，而由于结冷胶网络在中性条件下解体而从水凝胶状态转变为溶液状态。此外，基于双乳液填充水凝胶的 pH 敏感性，藻蓝蛋白和虾青素的共同包封和证明了体外释放行为。藻蓝蛋白和虾青素的最大包封率分别为94.1%和90.82%。体外消化证明了它们的肠道靶向释放能力。这些结果表明，双乳液填充的结冷胶水凝胶可用作 pH 敏感载体，用于肠道靶向递送疏水性和亲水性食品生物活性成分。