Background: Tanshinone IIA (Tan IIA) exerts a significant inhibitory effect on various tumor cells since it induces cell apoptosis and affects the proliferation, differentiation, metastasis, and invasion of tumor cells. However, the mechanism underlying the antitumor activity of Tan IIA has not been totally elucidated. Objective: This study aimed to uncover the role of Tan IIA in colorectal cancer (CRC) and its potential mechanism of action. Method: Cell proliferation was assessed using CCK-8 and colony formation assays. Western blot analysis was carried out to detect the expression of related proteins. Cell apoptosis was assessed using flow cytometry. Furthermore, tumor size and tumor weight of CRC xenograft mice were recorded before and after Tan IIA treatment. The production of reactive oxygen species (ROS) was measured by a ROS kit. Result: The results revealed that Tan IIA induced autophagy and apoptosis via activating the ROS/JNK signaling pathway in CRC cells, thus inhibiting the progression of CRC in vivo. Conclusion: The aforementioned findings indicated that Tan IIA exerted an antiproliferative effect on CRC by inducing cell autophagy and apoptosis via activating the ROS/JNK signaling pathway. Therefore, Tan IIA may be considered a potential therapeutic agent for treating CRC.

中文翻译：

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丹参酮 IIA 通过激活 ROS/JNK 信号通路减轻结直肠癌的生物学特性

背景：丹参酮ⅡA（TanⅡA）通过诱导细胞凋亡，影响肿瘤细胞的增殖、分化、转移和侵袭，对多种肿瘤细胞具有显着的抑制作用。然而，Tan IIA 抗肿瘤活性的潜在机制尚未完全阐明。目的：本研究旨在揭示 Tan IIA 在结直肠癌 (CRC) 中的作用及其潜在的作用机制。方法：使用 CCK-8 和集落形成测定评估细胞增殖。进行Western blot分析以检测相关蛋白的表达。使用流式细胞术评估细胞凋亡。此外，在 Tan IIA 治疗前后记录了 CRC 异种移植小鼠的肿瘤大小和肿瘤重量。通过 ROS 试剂盒测量活性氧 (ROS) 的产生。结果：结果表明，Tan IIA 通过激活 CRC 细胞中的 ROS/JNK 信号通路诱导自噬和细胞凋亡，从而在体内抑制 CRC 的进展。结论：上述研究结果表明，Tan IIA 通过激活 ROS/JNK 信号通路诱导细胞自噬和凋亡，从而对 CRC 发挥抗增殖作用。因此，Tan IIA 可能被认为是治疗 CRC 的潜在治疗剂。