Antioxidant and anti-inflammatory activities of Ficus awkeotsang Makino extract (FAE) on Hs68 fibroblasts and BALB/c nude-mouse models are evaluated in this study. FAE was found to be non-toxic and showed high levels of DPPH, H₂O₂, and hydroxyl radical scavenging abilities; a ferrous chelating capacity; as well as ferric-reducing antioxidant capability. The antioxidant activity of FAE was strongly associated with polyphenolic content (flavonoids at 10.3 mg QE g⁻¹ and total phenol at 107.6 mg GAE g⁻¹). The anti-inflammatory activity of FAE and the underlying molecular mechanisms were also investigated. The a* value of the mouse dorsal skin after treatment with FAE at 1.5 mg/mL in addition to chronic UVB exposure was found to decrease by 19.2% during a ten-week period. The anti-inflammatory effect of FAE was evidenced by the decreased accumulation of inflammatory cells and skin thickness. Expression levels of UVB-induced inflammatory proteins, including ROS, NF-κB, iNOS, COX-2, and IL-6, were significantly reduced upon FAE treatment in vitro and in vivo. Collectively, our results suggest that the inhibition of ROS and UVB-induced activation of the NF-κB downstream signaling pathway by FAE, indicating considerable potential as a versatile adjuvant against free radical damage in pharmaceutical applications.

中文翻译：

---

果冻无花果（Ficus awkeotsang Makino）通过 NFκB 信号通路调节活性氧的产生而表现出抗氧化和抗炎活性

本研究评估了Ficus awkeotsang Makino 提取物 (FAE) 对 Hs68 成纤维细胞和 BALB/c 裸鼠模型的抗氧化和抗炎活性。FAE 被发现是无毒的，并显示出高水平的 DPPH、H ₂ O ₂和羟基自由基清除能力；亚铁螯合能力；以及降低铁的抗氧化能力。FAE 的抗氧化活性与多酚含量密切相关（10.3 mg QE g ^-1的黄酮类化合物和 107.6 mg GAE g ^{-1的总酚）}）。还研究了 FAE 的抗炎活性和潜在的分子机制。发现在 10 周期间，除了慢性 UVB 暴露外，用 1.5 mg/mL 的 FAE 治疗后小鼠背部皮肤的 a* 值下降了 19.2%。FAE 的抗炎作用通过减少炎症细胞的积累和皮肤厚度得到证明。在体外和体内 FAE 处理后，UVB 诱导的炎症蛋白（包括 ROS、NF-κB、iNOS、COX-2 和 IL-6）的表达水平显着降低。总的来说，我们的结果表明 FAE 抑制 ROS 和 UVB 诱导的 NF-κB 下游信号通路激活，表明在药物应用中作为抗自由基损伤的多功能佐剂具有相当大的潜力。