Tus is a protein involved in DNA replication termination that binds specific DNA sequences (Ter) located around the terminus region of the chromosome in Enterobacterales. Tus and Ter form a unique monomeric protein-DNA complex which is one of strongest of its kind. A fascinating aspect of Tus-Ter is its ability to dramatically change conformation into a locked structure upon progression of a replication fork towards the non-permissive face of the complex. Over the last two decades, several new technologies have emerged harnessing the unique and interesting properties of this fascinating DNA-binding protein. This review highlights the important properties of the Tus-Ter complex and their exploitation for the development of diverse and novel ultrasensitive detection devices as well as innovative genomic and proteomic platform technologies. A variety of ex vivo and in vivo bioanalytical applications are discussed, including immuno-PCR diagnostic, bioassay and protein array technologies that are broadly relevant to the fields of cancer biology, microbiology and immunology. A perspective on future research and applications is provided.

Tus 是一种参与 DNA 复制终止的蛋白质，它与位于肠杆菌属染色体末端区域周围的特定 DNA 序列 ( Ter ) 结合。Tus 和Ter形成独特的单体蛋白质-DNA 复合物，是同类中最强的一种。Tuster 的一个令人着迷的方面是它能够在复制叉向复合体的非允许面前进时将构象显着改变为锁定结构。在过去的二十年里，利用这种迷人的 DNA 结合蛋白独特而有趣的特性，出现了几项新技术。这篇综述突出了 Tuster 的重要特性复杂性及其开发用于开发多样化和新颖的超灵敏检测设备以及创新的基因组和蛋白质组学平台技术。讨论了各种离体和体内生物分析应用，包括与癌症生物学、微生物学和免疫学领域广泛相关的免疫 PCR 诊断、生物测定和蛋白质阵列技术。提供了对未来研究和应用的展望。