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2-Methoxy-1,4-naphthoquinone regulated molecular alternation of Fusarium proliferatum revealed by high-dimensional biological data
RSC Advances ( IF 3.9 ) Pub Date : 2022-05-17 , DOI: 10.1039/d2ra02425j Jiajia Yang 1 , Xuewei Xia 1 , Meixia Guo 2 , Li Zhong 1 , Xiaoyong Zhang 3 , Xuewu Duan 4 , Jun Liu 5 , Riming Huang 1
RSC Advances ( IF 3.9 ) Pub Date : 2022-05-17 , DOI: 10.1039/d2ra02425j Jiajia Yang 1 , Xuewei Xia 1 , Meixia Guo 2 , Li Zhong 1 , Xiaoyong Zhang 3 , Xuewu Duan 4 , Jun Liu 5 , Riming Huang 1
Affiliation
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Fungi Fusarium proliferatum and the toxins it produces are hazardous to agricultural plants, animals, and human health. However, there is a lack of more effective and environment-friendly natural anti-F. proliferatum agents. In the search for natural anti-fungal agents, we found that naturally originated 2-methoxy-1,4-naphthoquinone (MNQ) with a minimal inhibitory dose of 8.0 mg L−1 possessed a potential inhibitory effect on F. proliferatum. The results of transcriptomic, proteomic, and metabolomic reveal a total of 1314 differential expression genes (DEGs, 873 up-regulated and 441 down-regulated), 259 differential expression proteins (DEPs, 104 up-regulated and 155 down-regulated), and 86 differential accumulation metabolites (DAMs, 49 up-regulated and 37 down-regulated) in MNQ-induced F. proliferatum. Further, the correlation analysis of transcriptomic, proteomic, and metabolomic indicated that these DEGs, DEPs, and DAMs were co-mapped in the pathways of glyoxylate and dicarboxylate metabolism, glycine, serine, and threonine metabolism, and pyruvate metabolism that linked to the TCA cycle. Furthermore, the key DEGs of the significantly co-mapped pathways were verified with qPCR analysis, which was related to the permeability of the cell membrane of F. proliferatum. Thus, these findings will provide fundamental scientific data on the molecular shifts of MNQ-induced F. proliferatum.
中文翻译：
高维生物学数据揭示2-甲氧基-1,4-萘醌调控增殖镰孢的分子变化
真菌Fusarium proliferatum及其产生的毒素对农业植物、动物和人类健康有害。然而，缺乏更有效和环境友好的天然抗增殖F.proliferatum药剂。在寻找天然抗真菌剂时，我们发现天然来源的 2-methoxy-1,4-naphthoquinone (MNQ)，最小抑制剂量为 8.0 mg L -1对F. proliferatum具有潜在的抑制作用. 转录组学、蛋白质组学和代谢组学的结果显示，共有 1314 个差异表达基因（DEG，873 个上调和 441 个下调），259 个差异表达蛋白（DEP，104 个上调和 155 个下调），以及MNQ 诱导的F. proliferatum中的 86 种差异积累代谢物（DAM，49 种上调和 37 种下调）. 此外，转录组学、蛋白质组学和代谢组学的相关分析表明，这些 DEGs、DEPs 和 DAMs 在与 TCA 相关的乙醛酸和二羧酸代谢、甘氨酸、丝氨酸和苏氨酸代谢以及丙酮酸代谢的途径中被共同映射。循环。此外，通过qPCR分析验证了显着共映射途径的关键DEG，这与F. proliferatum细胞膜的通透性有关。因此，这些发现将为 MNQ 诱导的F. proliferatum的分子转变提供基础科学数据。
更新日期：2022-05-17
中文翻译：
高维生物学数据揭示2-甲氧基-1,4-萘醌调控增殖镰孢的分子变化
真菌Fusarium proliferatum及其产生的毒素对农业植物、动物和人类健康有害。然而，缺乏更有效和环境友好的天然抗增殖F.proliferatum药剂。在寻找天然抗真菌剂时，我们发现天然来源的 2-methoxy-1,4-naphthoquinone (MNQ)，最小抑制剂量为 8.0 mg L -1对F. proliferatum具有潜在的抑制作用. 转录组学、蛋白质组学和代谢组学的结果显示，共有 1314 个差异表达基因（DEG，873 个上调和 441 个下调），259 个差异表达蛋白（DEP，104 个上调和 155 个下调），以及MNQ 诱导的F. proliferatum中的 86 种差异积累代谢物（DAM，49 种上调和 37 种下调）. 此外，转录组学、蛋白质组学和代谢组学的相关分析表明，这些 DEGs、DEPs 和 DAMs 在与 TCA 相关的乙醛酸和二羧酸代谢、甘氨酸、丝氨酸和苏氨酸代谢以及丙酮酸代谢的途径中被共同映射。循环。此外，通过qPCR分析验证了显着共映射途径的关键DEG，这与F. proliferatum细胞膜的通透性有关。因此，这些发现将为 MNQ 诱导的F. proliferatum的分子转变提供基础科学数据。
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