Development of biocompatible hydrogel adhesives with robust tissue adhesion to realize instant hemorrhage control and injury sealing, especially for emergency rescue and tissue repair, is still challenging. Herein, we report a potent hydrogel adhesive by free radical polymerization of N-acryloyl aspartic acid (AASP) in a facile and straightforward way. Through delicate adjustment of steric hindrance, the synergistic effect between interface interactions and cohesion energy can be achieved in PAASP hydrogel verified by X-ray photoelectron spectroscopy (XPS) analysis and simulation calculation compared to poly (N-acryloyl glutamic acid) (PAGLU) and poly (N-acryloyl amidomalonic acid) (PAAMI) hydrogels. The adhesion strength of the PAASP hydrogel could reach 120 kPa to firmly seal the broken organs to withstand the external force with persistent stability under physiological conditions, and rapid hemostasis in different hemorrhage models on mice is achieved using PAASP hydrogel as physical barrier. Furthermore, the paper-based Fe³⁺ transfer printing method is applied to construct PAASP-based Janus hydrogel patch with both adhesive and non-adhesive surfaces, by which simultaneous wound healing and postoperative anti-adhesion can be realized in gastric perforation model on mice. This advanced hydrogel may show vast potential as bio-adhesives for emergency rescue and tissue/organ repair.

开发具有强大组织粘附力的生物相容性水凝胶粘合剂以实现即时出血控制和损伤封闭，特别是用于紧急救援和组织修复，仍然具有挑战性。在此，我们报道了一种通过自由基聚合N-丙烯酰天冬氨酸 (AASP) 以一种简单直接的方式实现的有效水凝胶粘合剂。通过X射线光电子能谱（XPS）分析和模拟计算验证与聚（N-丙烯酰谷氨酸）（PAGLU）和聚 ( N-丙烯酰氨基丙二酸）（PAAMI）水凝胶。PAASP水凝胶的粘附强度可以达到120 kPa，牢固地密封破损器官以承受外力，在生理条件下具有持久的稳定性，并以PAASP水凝胶为物理屏障实现了小鼠不同出血模型的快速止血。此外，采用纸基Fe ³⁺转移印刷方法构建了具有粘性和非粘性表面的PAASP基Janus水凝胶贴片，可在小鼠胃穿孔模型中实现伤口同时愈合和术后抗粘连。 . 这种先进的水凝胶可能显示出作为生物粘合剂用于紧急救援和组织/器官修复的巨大潜力。