In current work, one novel benzothiazole-based fluorescent chemosensor (TZ-BO) with ESIPT luminescence mechanism was designed for the detection of hydrogen peroxide (H₂O₂) as typical reactive oxygen species (ROS). The boronate catenary in our designed probe molecule TZ-BO is both the blocking group of ESIPT and the reactive group of H₂O₂. The synthesized probe TZ-BO has the advantages of large Stokes shift, high sensitivity, good selectivity, and fast reaction speed to H₂O₂. The ability of the probe to detect H₂O₂ in aqueous media and exogenous H₂O₂ in cells by fluorescence signal was investigated. The experimental results manifested that probe TZ-BO is able to sensitively detect H₂O₂ using a fluorescence method in an excellent linear range of H₂O₂ concentration from 0 to 60 μM (R² = 0.994). The detection limit was determined as 1.0 × 10⁻⁶ M. Most importantly, the probe TZ-BO with highly low cytotoxicity can effectively detect exogenous H₂O₂ in HeLa cells. This probe provides a promising tool for monitoring the real concentration level of H₂O₂ in related physiological and pathological researches.

在目前的工作中，设计了一种具有 ESIPT 发光机制的新型苯并噻唑基荧光化学传感器 ( TZ-BO )，用于检测作为典型活性氧 (ROS)的过氧化氢 (H ₂ O _{2 )。}我们设计的探针分子TZ-BO中的硼酸盐链既是 ESIPT 的封闭基团，也是 H ₂ O ₂的反应基团。合成的探针TZ-BO具有斯托克斯位移大、灵敏度高、选择性好、对H ₂ O ₂反应速度快等优点。探针检测水介质中H ₂ O _{2和外源 H 的能力通过荧光信号研究了细胞中的2} O ₂。实验结果表明，探针TZ-BO能够在H ₂ O ₂浓度从0到60 μM（R ²  = 0.994）的良好线性范围内使用荧光法灵敏地检测H ₂ O _{2 。}检测限确定为1.0×10 ^-6  M。最重要的是，具有极低细胞毒性的探针TZ-BO可以有效检测HeLa细胞中的外源H ₂ O ₂。该探针为监测 H ₂ O _{2的实际浓度水平提供了一个有前途的工具}从事相关的生理和病理研究。