<br>细胞色素 P450 的表征在确定玉米除草剂选择性中起关键作用,ACS Omega

当前位置： X-MOL 学术 › ACS Omega › 论文详情

Our official English website, www.x-mol.net, welcomes your feedback! (Note: you will need to create a separate account there.)

细胞色素 P450 的表征在确定玉米除草剂选择性中起关键作用
ACS Omega ( IF 3.7 ) Pub Date : 2022-05-11 , DOI: 10.1021/acsomega.2c01705
Melissa Brazier-Hicks _{1,

2} , Sara Franco-Ortega ₁ , Philip Watson ₁ , Blandine Rougemont ₂ , Jonathan Cohn ₃ , Richard Dale ₂ , Tim R Hawkes ₂ , Alina Goldberg-Cavalleri ₁ , Nawaporn Onkokesung ₁ , Robert Edwards ₁

Affiliation

美卡米芬和苯诺沙考等安全剂广泛用于玉米，通过诱导关键解毒酶，特别是细胞色素 P450 单加氧酶 (CYP) 来增强除草剂的选择性。通过结合转录组学、蛋白质组学和功能测定，已经确定了在这种全球重要作物中负责除草剂代谢的安全剂诱导的 CYP。总共 18 个属于 71、72、74 和 86 族的 CYP 被安全剂诱导，各自的酶在酵母中表达，并筛选对噻二嗪（苯达松）、磺酰脲（烟嘧磺隆）和三酮（甲基磺草酮和环磺草酮）的活性化学。除草剂代谢主要限于第 71 族的 CYP81A 家族成员，特别是 CYP81A9、CYP81A16 和 CYP81A2。定量转录组学和蛋白质组学表明，CYP81A9/CYP81A16 是安全剂处理的大田玉米中的优势酶，而在甜玉米中仅检测到 CYP81A9。通过将 CYP81A2 和 CP81A9 剪接在一起作为一系列重组嵌合体，研究了 CYP81A 序列和活性之间的关系。 CYP81A9 对三种除草剂化学物质表现出广泛的活性，而 CYP81A2 在多个位置独特地羟基化苯达松。 CYP81A9 对多种除草剂的底物特异性的可塑性位于其 N 末端一半的第二个四分位。 CYP81A9 的进一步系统发育分析表明，玉米酶与其他与谷物和野草农化代谢相关的 CYP81A 相关，表明 71 CYP 族在确定耕作作物除草剂选择性方面具有独特的功能。

"点击查看英文标题和摘要"

Characterization of Cytochrome P450s with Key Roles in Determining Herbicide Selectivity in Maize

Safeners such as metcamifen and benoxacor are widely used in maize to enhance the selectivity of herbicides through the induction of key detoxifying enzymes, notably cytochrome P450 monooxygenases (CYPs). Using a combination of transcriptomics, proteomics, and functional assays, the safener-inducible CYPs responsible for herbicide metabolism in this globally important crop have been identified. A total of 18 CYPs belonging to clans 71, 72, 74, and 86 were safener-induced, with the respective enzymes expressed in yeast and screened for activity toward thiadiazine (bentazon), sulfonylurea (nicosulfuron), and triketone (mesotrione and tembotrione) chemistries. Herbicide metabolism was largely restricted to family CYP81A members from clan 71, notably CYP81A9, CYP81A16, and CYP81A2. Quantitative transcriptomics and proteomics showed that CYP81A9/CYP81A16 were dominant enzymes in safener-treated field maize, whereas only CYP81A9 was determined in sweet corn. The relationship between CYP81A sequence and activities were investigated by splicing CYP81A2 and CP81A9 together as a series of recombinant chimeras. CYP81A9 showed wide ranging activities toward the three herbicide chemistries, while CYP81A2 uniquely hydroxylated bentazon in multiple positions. The plasticity in substrate specificity of CYP81A9 toward multiple herbicides resided in the second quartile of its N terminal half. Further phylogenetic analysis of CYP81A9 showed that the maize enzyme was related to other CYP81As linked to agrochemical metabolism in cereals and wild grasses, suggesting this clan 71 CYP has a unique function in determining herbicide selectivity in arable crops.

更新日期：2022-05-11

点击分享查看原文

点击收藏

阅读更多本刊新发论文本刊介绍/投稿指南