New Approach to Generate Ratiometric Signals on Immunochromatographic Strips for Small Molecules,Analytical Chemistry

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New Approach to Generate Ratiometric Signals on Immunochromatographic Strips for Small Molecules
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-05-10 , DOI: 10.1021/acs.analchem.2c00838
He Chen _{1,

2} , Yuan Ding _{1,

2} , Jiao Li _{1,

2} , Lianrun Huang _{1,

2} , Gualberto González-Sapienza ₃ , Bruce D Hammock ₄ , Minghua Wang _{1,

2} , Xiude Hua _{1,

2}

Affiliation

The self-calibration capability of ratiometric signals has been widely considered to enhance the accuracy, sensitivity, and anti-interference ability of immunoassays. Exploring a new approach to generate ratiometric signals can provide more options for various requirements. Herein, we integrated the negative-readout competitive and positive-readout noncompetitive immunoassays into a single assay by employing different color tracers, labeled peptidomimetic and anti-immunocomplex peptides, to create a new unconstrained ratiometric signal approach. Using an immunochromatographic strip (ICS) and a fungicide benzothiostrobin as the analytical platform and analyte, respectively, we showed that this approach can be extensively applied to fluorescence and colorimetry readouts, which have also been proven for strong anti-interference ability to an external light environment. Moreover, the enormous intuitional color changes of ratiometric fluorescent and colorimetric ICSs (RFICS and RCICS) enabled the formation of the color reference cards (like the pH paper) for visual judgment. After adaptation with a portable smartphone, the quantitative detection limits for RFICS and RCICS were 0.17 and 0.44 ng mL^–1, respectively. In addition, the ICSs showed good accuracy for the detection of benzothiostrobin in spiked samples.

中文翻译：

在小分子免疫层析条上生成比率信号的新方法

比率信号的自校准能力已被广泛认为可以提高免疫测定的准确性、灵敏度和抗干扰能力。探索一种生成比率信号的新方法可以为各种需求提供更多选择。在这里，我们通过采用不同的颜色示踪剂、标记的肽模拟物和抗免疫复合物肽，将负读出竞争性和正读出非竞争性免疫测定整合到一个单一测定中，以创建一种新的无约束比率信号方法。分别使用免疫层析条 (ICS) 和杀菌剂苯并硫醚菌酯作为分析平台和分析物，我们表明这种方法可以广泛应用于荧光和比色读数，也被证明对外部光环境具有很强的抗干扰能力。此外，比率荧光和比色 ICS（RFICS 和 RCICS）的巨大直观颜色变化使得能够形成用于视觉判断的颜色参考卡（如 pH 试纸）。与便携式智能手机适配后，RFICS 和 RCICS 的定量检测限分别为 0.17 和 0.44 ng mL^–1，分别。此外，ICS 对加标样品中苯并硫醚菌酯的检测显示出良好的准确性。

更新日期：2022-05-10

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