In the last years, peptide-based hydrogels are being increasingly used as suitable matrices for biomedical and pharmaceutical applications, including drug delivery and tissue engineering. Recently, the synthesis and the gelation properties of a small library of cationic peptides, containing a Lys residue at the C-terminus and derivatized with an Fmoc group or with the fluorenyl methoxycarbonyl-diphenylalanine (FmocFF) at the N-terminus are derived. Here, it is demonstrated that the combination of these peptides with the well-known hydrogelator FmocFF, in different weight/weight ratios, allows the achievement of seven novel self-sorted hydrogels, which share similar peptide organization of their supramolecular matrix. Rheological and relaxometric characterization highlight a different mechanical rigidity and water mobility in the gels as demonstrated by the storage modulus values (200 Pa < G’ < 35 000 Pa) and by relaxometry, respectively. In vitro studies demonstrate that most of the tested mixed hydrogels do not disturb significantly the cell viability (>95%) over 72 h of treatment. Moreover, in virtue to its capability to strongly favor adhesion, spreading and duplication of 3T3-L1 cells, one of the tested hydrogel may be eligible as synthetic extracellular matrix.

中文翻译：

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Fmoc-FF 和阳离子肽的多组分水凝胶基质在组织工程中的应用

在过去几年中，基于肽的水凝胶越来越多地用作生物医学和药物应用的合适基质，包括药物递送和组织工程。最近，衍生了一个小型阳离子肽库的合成和凝胶特性，该库在 C 端含有赖氨酸残基，并在 N 端用 Fmoc 基团或芴基甲氧羰基-二苯丙氨酸 (FmocFF) 衍生。在这里，证明了这些肽与众所周知的水凝胶剂 FmocFF 以不同的重量/重量比组合，可以实现七种新型自分类水凝胶，它们具有相似的超分子基质肽结构。G' < 35 000 Pa) 和弛豫测量法。体外研究表明，大多数测试的混合水凝胶在处理 72 小时后不会显着干扰细胞活力 (>95%)。此外，由于其强烈支持 3T3-L1 细胞的粘附、扩散和复制的能力，一种测试的水凝胶可能有资格作为合成细胞外基质。