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Chloroplast genome of the invasive Pyrus calleryana underscores the high molecular diversity of the species
Journal of Applied Genetics ( IF 2.0 ) Pub Date : 2022-05-06 , DOI: 10.1007/s13353-022-00699-8
Marcin Nowicki 1 , Matthew L Huff 1 , Margaret E Staton 1 , Robert N Trigiano 1
Affiliation  

The complete chloroplast genome of Pyrus calleryana (GenBank OM541581.1) was developed by de novo assembly from whole-genome sequencing data. Reference-guided (P. phaeocarpa) read mapping and assembly were followed by annotation and phylogenetic comparisons. The 159,965 bp P. calleryana chloroplast genome represented 36.56% GC content with a classical quadripartite architecture and two inverted repeats regions (IRs; each 26,392 bp) separating the large single-copy region (LSC; 87,942 bp) and the small single-copy region (SSC; 19.239 bp). In total, 125 unique features were annotated in that genome, including 83 protein coding genes, 38 tRNA coding genes, and 4 rRNA coding genes. Phylogenetic analyses based on the whole chloroplast genome sequences placed the P. calleryana among other Rosaceae plants, specifically among the Asian species of Pyrus.



中文翻译:

侵入性 Pyrus calleryana 的叶绿体基因组强调了该物种的高分子多样性

Pyrus calleryana (GenBank OM541581.1)的完整叶绿体基因组是通过全基因组测序数据的从头组装开发的。参考引导 ( P. phaeocarpa ) 读取映射和组装之后是注释和系统发育比较。159,965 bp 的P. calleryana叶绿体基因组代表 36.56% 的 GC 含量,具有经典的四分结构和两个反向重复区域(IR;每个 26,392 bp)将大单拷贝区域(LSC;87,942 bp)和小单拷贝区域分开(SSC;19.239 bp)。在该基因组中总共注释了 125 个独特的特征,包括 83 个蛋白质编码基因、38 个 tRNA 编码基因和 4 个 rRNA 编码基因。基于全叶绿体基因组序列的系统发育分析P. calleryana在其他蔷薇科植物中,特别是在亚洲属植物中。

更新日期:2022-05-09
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