Phlomis Medicinalis Diels 中的环烯醚萜苷：优化提取和止血评估,Chemistry & Biodiversity

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Phlomis Medicinalis Diels 中的环烯醚萜苷：优化提取和止血评估
Chemistry & Biodiversity ( IF 2.3 ) Pub Date : 2022-05-03 , DOI: 10.1002/cbdv.202100936
Sitong Lu ₁ , Zhaoyu Zhang ₁ , Youling Liang ₁ , Qingkun Zhong ₁ , Guangfa Li ₁ , Zhang Hu ₁

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桑葚是青藏高原特有的重要多年生草本植物，常被用作藏药桑葚，用于治疗感冒、咳嗽、收敛创伤。为了有效地从药材上提取环烯醚萜苷，采用超声辅助深共熔溶剂萃取技术。通过单因素试验研究了影响提取过程的主要参数，并采用响应面法对提取进行了优化。体外评价了药用假单胞菌总环烯醚萜苷 (TIG) 的止血活性在老鼠身上。优化结果表明,最佳工艺参数为液固比20:1、氯化胆碱-乳酸浓度79%、超声时间34 min,TIG萃取得率达到20.73 %。同时，采用高效液相色谱-光电二极管阵列/质谱（HPLC-PDA/MS）对优化后的提取物进行表征，表明药用紫菀的TIG主要由16种已报道的环烯醚萜苷组成，总含量为91.22%。体内和体外实验结果表明药用假单胞菌的 TIG具有很强的止血活性，这可以通过增加纤维蛋白原水平来实现。因此，超声辅助深度低共熔溶剂萃取是从药用白芍中提取环烯醚萜苷的有效方法，将成为有希望开发的医用止血剂候选药物。

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Iridoid Glycosides from Phlomis Medicinalis Diels: Optimized Extraction and Hemostasis Evaluation

Phlomis medicinalis Diels, an important perennial herbal plant unique to the Qinghai-Tibet Plateau, is often used as Tibetan Materia Medicine Radix Phlomii for the treatment of cold, cough, and convergence trauma. In order to efficiently extract the iridoid glycosides from P. medicinalis, an ultrasound-assisted deep eutectic solvent extraction technique was employed. The main parameters influencing the extraction process were studied through single-factor tests and the extraction was optimized by using response surface methodology. The hemostasis activity of total iridoid glycosides (TIG) from P. medicinalis was evaluated in vitro and in mice. The optimization results revealed that the optimal process parameters were liquid-solid ratio 20 : 1, choline chloride-lactic acid concentration 79 %, and sonication time 34 min, under which a TIG extraction yield of 20.73 % was obtained. Meanwhile, high-performance liquid chromatography-photodiode array/mass spectrometry (HPLC-PDA/MS) was employed to characterize the optimized extract and indicated that TIG from P. medicinalis mainly consisted of sixteen reported iridoid glycosides with a total content of 91.22 %. The experimental results in vivo and in vitro indicated that TIG from P. medicinalis had strong hemostasis activities, which may be achieved by increasing the fibrinogen levels. Therefore, the ultrasound-assisted deep eutectic solvent extraction is an effective method to extract iridoid glycosides from P. medicinalis and they will be promising candidates to be developed for medical hemostasis agents.

更新日期：2022-05-03

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