Vitexin-2-O-rhamnoside (VR) is an important active substance in hawthorn, which is widely used as a food or functional food raw material; however, its immunomodulatory activities have not been extensively studied. In this study, BALB/c mice immunocompromised by cyclophosphamide (CY) were used as models to explore the effects of VR on the immunity and antioxidant capacity of mice. The results revealed that VR can restore weight to the immunosuppressed mice to varying degrees, improve spleen and thymus injury, and restore peripheral blood levels. Furthermore, it can effectively promote the proliferation of T and B lymphocytes, natural killer (NK) and cytotoxic T lymphocyte (CTL) cell activities, and the secretion and mRNA expression of cytokines IFN-γ, IL-2, IL-6, and IL-12 to 0.36, 0.34, 50.25%, 45.74%, 28.36 pg/mL or 0.68, 31.81 pg/mL or 0.74, 20.40 pg/mL or 0.75, and 19.81 pg/mL or 0.55, respectively. Moreover, it can upregulate the phosphorylation level of PI3K/Akt signaling pathway in mice immunosuppressed by CY, increase the activities of glutathione peroxidase (GSH-Px), chloramphenicol acetyltransferase (CAT), superoxide dismutase (SOD), and total antioxidant capacity (T-AOC), and decrease the level of malondialdehyde (MDA). This study provides a theoretical and experimental basis for the research and development of health products with targeted efficacy, and the development of diversified products in the hawthorn deep-processing industry.

Vitexin-2-O-rhamnoside (VR)是山楂中的重要活性物质，被广泛用作食品或功能食品原料；然而，其免疫调节活性尚未得到广泛研究。本研究以环磷酰胺（CY）免疫功能低下的BALB/c小鼠为模型，探讨VR对小鼠免疫和抗氧化能力的影响。结果表明，VR可以不同程度地恢复免疫抑制小鼠的体重，改善脾脏和胸腺损伤，恢复外周血水平。此外，它还能有效促进 T 和 B 淋巴细胞的增殖、自然杀伤 (NK) 和细胞毒性 T 淋巴细胞 (CTL) 细胞的活性，以及​​细胞因子 IFN-γ、IL-2、IL-6 和IL-12 至 0.36、0.34、50.25%、45.74%、28.36 pg/mL 或 0.68、31.81 pg/mL 或 0.74、20。分别为 40 pg/mL 或 0.75，和 19.81 pg/mL 或 0.55。此外，它可以上调 CY 免疫抑制小鼠 PI3K/Akt 信号通路的磷酸化水平，增加谷胱甘肽过氧化物酶（GSH-Px）、氯霉素乙酰转移酶（CAT）、超氧化物歧化酶（SOD）和总抗氧化能力（T -AOC)，并降低丙二醛 (MDA) 的水平。本研究为山楂深加工行业针对性功效保健品的研发和多元化产品的开发提供理论和实验依据。氯霉素乙酰转移酶（CAT）、超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC），降低丙二醛（MDA）水平。本研究为山楂深加工行业针对性功效保健品的研发和多元化产品的开发提供理论和实验依据。氯霉素乙酰转移酶（CAT）、超氧化物歧化酶（SOD）和总抗氧化能力（T-AOC），降低丙二醛（MDA）水平。本研究为山楂深加工行业针对性功效保健品的研发和多元化产品的开发提供理论和实验依据。