Liamocins and Massoia lactone have many applications. In this study, the glucose-derepressed mutant Δcrea5 in which the CREA gene was removed could produce 36.5 g/L of liamocins. Furthermore, overexpression of the MSN2 gene in the mutant Δcrea5 made the transformant M60 produce 41.4 g/L of liamocins and further overexpression of the GAL1 gene in the transformant M60 rendered the transformant G40 to produce 49.5 ± 0.4 g/L of liamocins during the 10-L fermentation while their wild type strain 9–1 made only 26.3 g/L of liamocins. The expressed transcription activators Msn2 and Gal1 were localized in the nuclei, promoting expression of the genes responsible for liamocins biosynthesis and sugar transport. Massoia lactone prepared from the produced liamocins could actively kill the spores of the pathogenic fungi from the diseased human skin by inhibiting spore germination and causing cellular necrosis of the fungal spores.

Liamocins 和 Massoia lactone 有许多应用。在这项研究中，去除了CREA基因的葡萄糖去抑制突变体Δcrea5可以产生 36.5 g/L 的 liamocins。此外，突变体Δcrea5中MSN2基因的过表达使转化体 M60 产生 41.4 g/L 的 liamocins 并进一步过表达GAL1转化子 M60 中的基因使转化子 G40 在 10-L 发酵过程中产生 49.5 ± 0.4 g/L 的 liamocin，而其野生型菌株 9-1 仅产生 26.3 g/L 的 liamocin。表达的转录激活因子 Msn2 和 Gal1 定位在细胞核中，促进了负责 liamocins 生物合成和糖转运的基因的表达。用产生的liamocins制备的马索内酯可以通过抑制孢子萌发并引起真菌孢子细胞坏死来主动杀死患病人体皮肤中病原真菌的孢子。