Microchimica Acta ( IF 5.3 ) Pub Date : 2022-05-02 , DOI: 10.1007/s00604-022-05292-8 Anni Zhu 1 , Tiansheng Wang 1 , Yuning Jiang 1 , Sen Hu 1 , Wanxin Tang 1 , Xinling Liu 1 , Xiaoyu Guo 1 , Ye Ying 1 , Yiping Wu 1 , Ying Wen 1 , Haifeng Yang 1
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A specific surface-enhanced Raman scattering (SERS) assay for dopamine (DA) based on an azo derivatization reaction is proposed for the first time by preparation of p-aminothiophenol (PATP)-modified composite SERS substrate, composed of metal–organic framework (MIL-101) decorated with Au and Ag nanoparticles. As the result, the SERS method for detection of the azo reaction between PATP and DA exhibits superior sensitivity, selectivity, and stability. A reasonable linearity in the range 10−6 to 10−10 mol∙L−1 is achieved, and the limit of detection is 1.2 × 10−12 mol∙L−1. The reactive SERS assay is free from interference in complex physiological fluid. The feasibility of the proposed SERS method for the detection of DA levels in fetal bovine serum (FBS) samples and human serum samples is validated by HPLC–MS methods, displaying promising application potential in early disease diagnosis.
Graphical abstract
中文翻译：
用对氨基苯硫酚偶氮衍生后，用 Ag/Au 贵金属纳米颗粒复合材料修饰的金属有机骨架的 SERS 测定多巴胺
通过制备由金属-有机骨架组成的对氨基苯硫酚 (PATP) 修饰的复合 SERS 底物，首次提出了一种基于偶氮衍生反应的多巴胺 (DA) 表面增强拉曼散射 (SERS) 检测方法。 MIL-101) 装饰有金和银纳米粒子。因此，用于检测 PATP 和 DA 之间偶氮反应的 SERS 方法表现出优异的灵敏度、选择性和稳定性。实现了 10 -6至 10 -10 mol∙L -1范围内的合理线性，检测限为 1.2 × 10 -12 mol∙L -1. 反应性 SERS 测定不受复杂生理液的干扰。所提出的 SERS 方法检测胎牛血清 (FBS) 样品和人血清样品中 DA 水平的可行性通过 HPLC-MS 方法得到验证，在早期疾病诊断中显示出广阔的应用潜力。
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