Hydrazine has a wide range of industrial applications, but it is also a toxic and explosive chemical substance, which brings potential risks to human health and environmental safety. Therefore, rapid and sensitive monitoring of hydrazine is of great importance in environmental sciences and biological systems. In this work, a new near-infrared (NIR) ratiometric fluorescent probe (Ac-HY) was designed to detect hydrazine under double excitation and emission mode. Ac-HY exhibited large stokes shift (130 nm), high selectivity and sensitivity to hydrazine detection. The applications of Ac-HY probe for detecting hydrazine in vapor and imaging hydrazine in lipid droplets and zebrafish. Therefore, Ac-HY can be used to monitor the distribution of exogenous hydrazine in vitro and in vivo.

肼具有广泛的工业应用，但它也是一种有毒、易爆的化学物质，给人类健康和环境安全带来潜在风险。因此，肼的快速灵敏监测在环境科学和生物系统中具有重要意义。在这项工作中，设计了一种新的近红外（NIR）比率荧光探针（Ac-HY），用于在双激发和发射模式下检测肼。Ac-HY表现出大的斯托克斯位移 (130 nm)、高选择性和对肼检测的灵敏度。Ac-HY探针在检测蒸气中肼和成像脂滴和斑马鱼中的肼方面的应用。因此，Ac-HY可用于监测外源性肼在体外和体内的分布。