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Tetrahedral DNA Nanostructure with Multiple Target-Recognition Domains for Ultrasensitive Electrochemical Detection of Mucin 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-04-27 , DOI: 10.1021/acs.analchem.2c00864 Wenxi Hu 1 , Yuanyuan Chang 1 , Junqing Huang 1 , Yaqin Chai 1 , Ruo Yuan 1
Analytical Chemistry ( IF 6.7 ) Pub Date : 2022-04-27 , DOI: 10.1021/acs.analchem.2c00864 Wenxi Hu 1 , Yuanyuan Chang 1 , Junqing Huang 1 , Yaqin Chai 1 , Ruo Yuan 1
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In this work, a tetrahedral DNA nanostructure (TDN) designed with multiple biomolecular recognition domains (m-TDN) was assembled to construct an ultrasensitive electrochemical biosensor for the quantitative detection of tumor-associated mucin 1 (MUC-1) protein. This new nanostructure not only effectively increased the capture efficiency of target proteins compared to the traditional TDN with a single recognition domain but also enhanced the sensitivity of the constructed electrochemical biosensors. Once the target MUC-1 was captured by the protein aptamers, the ferrocene-marked DNA strands as electrochemical signal probes at the vertices of m-TDN would be released away from the electrode surface, causing significant reduction of the electrochemical signal, thereby enhancing significantly the detection sensitivity. As a result, this well-designed biosensor achieved ultrasensitive detection of the biomolecule at a linear range from 1 fg mL–1 to 1 ng mL–1, with the limit of detection down to 0.31 fg mL–1. This strategy provides a new approach to enhance the detection sensitivity for the diagnosis of diseases.
中文翻译：
具有多个目标识别域的四面体 DNA 纳米结构用于粘蛋白 1 的超灵敏电化学检测
在这项工作中，将设计有多个生物分子识别域 (m-TDN) 的四面体 DNA 纳米结构 (TDN) 组装成用于定量检测肿瘤相关粘蛋白 1 (MUC-1) 蛋白的超灵敏电化学生物传感器。与具有单一识别域的传统 TDN 相比，这种新的纳米结构不仅有效地提高了靶蛋白的捕获效率，而且提高了构建的电化学生物传感器的灵敏度。一旦目标 MUC-1 被蛋白质适体捕获，作为电化学信号探针的二茂铁标记的 DNA 链在 m-TDN 的顶点会从电极表面释放出来，导致电化学信号显着降低，从而显着增强检测灵敏度。因此，–1至 1 ng mL –1，检测限低至 0.31 fg mL –1。该策略为提高疾病诊断的检测灵敏度提供了一种新方法。
更新日期：2022-04-27
中文翻译：
具有多个目标识别域的四面体 DNA 纳米结构用于粘蛋白 1 的超灵敏电化学检测
在这项工作中，将设计有多个生物分子识别域 (m-TDN) 的四面体 DNA 纳米结构 (TDN) 组装成用于定量检测肿瘤相关粘蛋白 1 (MUC-1) 蛋白的超灵敏电化学生物传感器。与具有单一识别域的传统 TDN 相比，这种新的纳米结构不仅有效地提高了靶蛋白的捕获效率，而且提高了构建的电化学生物传感器的灵敏度。一旦目标 MUC-1 被蛋白质适体捕获，作为电化学信号探针的二茂铁标记的 DNA 链在 m-TDN 的顶点会从电极表面释放出来，导致电化学信号显着降低，从而显着增强检测灵敏度。因此，–1至 1 ng mL –1，检测限低至 0.31 fg mL –1。该策略为提高疾病诊断的检测灵敏度提供了一种新方法。
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