体外胃肠消化对红景天酚类成分、生物活性和生物可及性的影响,Food & Function

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体外胃肠消化对红景天酚类成分、生物活性和生物可及性的影响
Food & Function ( IF 5.1 ) Pub Date : 2022-04-27 , DOI: 10.1039/d2fo00469k
Sheng Zhang ₁ , Na Deng _{1,

2} , Bisheng Zheng _{1,

3} , Tong Li ₄ , Rui Hai Liu ₄

Affiliation

本研究首先评估了模拟体外消化后红景天的酚类成分以及抗氧化和抗增殖活性的变化。此外，还进行了渗透性和摄取测定以及 RT-qPCR 和蛋白质印迹分析，以探索食糜的生物可及性及其潜在机制。结果表明，体外胃肠消化显着降低了红景天的总酚类和总黄酮类物质以及细胞外、细胞抗氧化和抗增殖活性，其中结肠食糜的降低幅度最大。然而，体外消化增加了红景天的细胞摄取率酚醛树脂具有较高的渗透系数。结肠食糜 (GA-Dig) 对没食子酸 (GA，主要化合物) 的摄取量最高，而不是纯 GA，表明 GA 和其他酚类物质在红景天中具有协同作用。深入的机制研究表明，结肠食糜的惊人摄取率和渗透系数是由 ABCF2 mRNA 和蛋白质表达水平的下调引发的。这些发现表明，模拟胃肠消化可以促进红景天酚类物质的生物可及性和生物活性。

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The effect of in vitro gastrointestinal digestion on the phenolic profiles, bioactivities and bioaccessibility of Rhodiola

Changes in the phenolic profiles and antioxidant and antiproliferative activities of Rhodiola after simulated in vitro digestion were first assessed in this study. Furthermore, permeability and uptake assays as well as RT-qPCR and western blot analyses were performed in order to explore the bioaccessibility of the digesta and its underlying mechanism. The results reveal that in vitro gastrointestinal digestion significantly reduced the total phenolics and total flavonoids as well as the extracellular, cellular antioxidant and antiproliferative activities of Rhodiola, in which the colon digesta had the largest reduction. However, in vitro digestion augmented the cellular uptake rates of Rhodiola phenolics with higher permeability coefficients. The colon digesta (GA-Dig) exhibited the highest uptake of gallic acid (GA, the main compound) instead of GA in its pure form, indicating the synergistic effects of GA and other phenolics in Rhodiola. In-depth mechanistic studies suggest that the fabulous uptake rates and permeability coefficients of the colon digesta were triggered by the down-regulation of the expression levels of ABCF2 mRNA and protein. These findings indicate that simulated gastrointestinal digestion could promote the bioaccessibility and bioactivities of phenolics in Rhodiola.

更新日期：2022-04-27

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