The simultaneous analysis of amino acids (AAs) is crucial for human health, diagnosis and treatment of disease, and nutritional quality evaluation in foodstuffs. Here, we establish an easy and rapid method for the simultaneous analysis of AAs using a single reagent 2-(trifluoromethyl)benzaldehyde (oTFMBA) based on spectral-separation-enabled ¹⁹F NMR spectroscopy. oTFMBA, a highly sensitive chemosensor, is capable of analyzing 19 proteinogenic AAs or non-amino acid amines (non-AAs) in a complex mixture by adjusting the pH in a toilless way. The ¹⁹F signals of oTFMBA-labeled AAs are distributed over a wide range of ∼ 0.7 ppm, demonstrating oTFMBA with higher resolution for simultaneous analysis of AAs compared to the o-phthaldialdehyde (OPA) method (<0.6 ppm). Additionally, 12 AAs were unambiguously identified in human urine, including Asp, Ser, Gly, Thr, Glu, Arg, Ala, Val, Ile, Tyr, His, and Phe. Furthermore, our method’s detection limit for AAs is 5.83 μM, illustrating sensitivity with an ∼100-fold improvement over the OPA method. This work represents an approach to the analysis of AAs or non-AAs in a complicated mixture (even biofluid) using a ¹⁹F NMR probe with high sensitivity, which is of great significance for the simultaneous analysis of multiple analytes.

氨基酸 (AA) 的同步分析对于人类健康、疾病的诊断和治疗以及食品中的营养质量评估至关重要。在这里，我们建立了一种使用单一试剂 2-(三氟甲基)苯甲醛 ( o TFMBA) 基于光谱分离的¹⁹ F NMR 光谱同时分析 AA 的简单快速的方法。o TFMBA 是一种高度敏感的化学传感器，能够通过轻松调节 pH 值来分析复杂混合物中的 19 种蛋白质氨基酸或非氨基酸胺（非氨基酸）。o的^19个F信号TFMBA 标记的 AA 分布在约 0.7 ppm 的宽范围内，表明 oTFMBA 与邻苯二甲醛 (OPA) 方法（<0.6 ppm）相比具有更高的分辨率，可同时分析 AA。此外，在人尿中明确鉴定出 12 种氨基酸，包括 Asp、Ser、Gly、Thr、Glu、Arg、Ala、Val、Ile、Tyr、His 和 Phe。此外，我们的方法对 AA 的检测限为 5.83 μM，说明灵敏度比 OPA 方法提高了 100 倍。^{这项工作代表了一种使用高灵敏度19} F NMR 探针分析复杂混合物（甚至生物流体）中的 AA 或非 AA 的方法，这对于同时分析多种分析物具有重要意义。