N-Alkylated chitosan derivative was synthesized by Michael addition reaction of chitosan and hydroxyethylacryl. The chemical structure and physical properties of the chitosan derivatives were characterized by FT-IR, 1H NMR, XRD and DSC techniques. The 1H NMR results indicated that the degree of substitution (DS) was from 0.18 to 1.2. The chitosan derivatives exhibited an excellent solubility in distilled water. XRD analysis showed that the derivatives were amorphous. DSC results demonstrated that thermal stability of the derivatives was lower than that of chitosan and it decomposed around 226 °C for hydroxyethylacryl-CS with DS higher than 1.05. The lysozyme enzymatic degradation results revealed that the chitosan derivatives had the ability to enzymatic degradation and the initial degradation rate of the chitosan derivatives was dependent on its molecular weight. The antimicrobial activity of the chitosan derivatives was decreased compared with that of chitosan, but it still had the ability of antimicrobial.

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水溶性N-烷基化壳聚糖的制备与表征

N-烷基化壳聚糖衍生物是通过壳聚糖与羟乙基丙烯酰基的迈克尔加成反应合成的。采用FT-IR、1H NMR、XRD和DSC技术对壳聚糖衍生物的化学结构和物理性质进行了表征。1H NMR结果表明取代度(DS)为0.18至1.2。壳聚糖衍生物在蒸馏水中表现出极好的溶解度。XRD分析表明衍生物是无定形的。DSC结果表明，该衍生物的热稳定性低于壳聚糖，羟乙基丙烯酰-CS在226℃左右分解，DS高于1.05。溶菌酶酶降解结果表明壳聚糖衍生物具有酶降解能力，壳聚糖衍生物的初始降解速率取决于其分子量。壳聚糖衍生物的抗菌活性较壳聚糖有所降低，但仍具有抗菌能力。