A novel fluorescent assay for uracil DNA glycosylase activity built on the 3′–5′ exonuclease activity-based endonuclease IV cyclic signal amplification strategy,New Journal of Chemistry

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A novel fluorescent assay for uracil DNA glycosylase activity built on the 3′–5′ exonuclease activity-based endonuclease IV cyclic signal amplification strategy
New Journal of Chemistry ( IF 2.7 ) Pub Date : 2020-01-01 , DOI: 10.1039/d0nj04729e
Jing-Xuan Tian _{1,

2,

3,

4} , Yan-Zhao Fang _{1,

2,

3,

4} , Yi-Xuan Yang _{1,

2,

3,

4} , Shuang Wu _{4,

5,

6,

7} , Qiang Xiao _{1,

2,

3,

4} , Xiang-Juan Kong _{1,

2,

3,

4}

Affiliation

The unique 3′-5′ exonuclease activity of endonuclease IV to DNA strands has been demonstrated, which enables the development of a novel highly sensitive assay for UDG activity.

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