Abstract Enantioseparation of racemic tryptophan is very important for pharmaceutical production. Conventional separation methods are high costly, energy intensive and environmentally unfriendly. Permeation separation based on membrane separation technique is a promising technology with advantages of energy efficient, clean and no additives. However, lots of organic compounds are commonly used for the traditional preparation of various membranes for enantioseparation of racemic tryptophan. Thus, we developed a totally green and clean preparation method using nature polymer sodium alginate as functional polymer, water as solvent and CaCl2 as crosslinking agent. Besides, polyvinylidene fluoride membrane was selected as supported membrane. Molecularly imprinted technology with d -Tryptophan as template molecule was introduced to enlarge mass transfer flux. Various methods were used in characterization of molecularly imprinted composite membrane (MICM) and non-imprinted composite membrane (NICM). Effects of preparing conditions, permeation conditions and concentration polarization on permeation performance of MICM by pressure-driven were investigated. High enantiomeric excess (ee% > 98%) of the permeation solution could be obtained under mild conditions. Reducing the presence of concentration polarization was beneficial to maintaining high ee% (>98%). Moreover, the thermo and chemical stability of MICM were studied too. Comparison with previous studies indicated that the proposed totally green and clean method was not only low costly and environment friendly but also beneficial to significantly increasing ee.

中文翻译：

---

外消旋色氨酸d-色氨酸印迹复合膜的绿色制备及选择性渗透

摘要 外消旋色氨酸的对映体分离在药物生产中具有重要意义。传统的分离方法成本高、能源密集且对环境不友好。基于膜分离技术的渗透分离技术具有节能、清洁、无添加剂等优点。然而，许多有机化合物通常用于传统的各种膜的制备，用于外消旋色氨酸的对映体分离。因此，我们开发了一种以天然聚合物海藻酸钠为功能聚合物、水为溶剂、CaCl2为交联剂的完全绿色、清洁的制备方法。此外，选择聚偏二氟乙烯膜作为支撑膜。引入以d-色氨酸为模板分子的分子印迹技术，扩大传质通量。各种方法用于表征分子印迹复合膜 (MICM) 和非印迹复合膜 (NICM)。考察了制备条件、渗透条件和浓差极化对MICM压力驱动渗透性能的影响。在温和条件下可以获得高对映体过量（ee% > 98%）的渗透溶液。减少浓差极化的存在有利于保持高 ee% (>98%)。此外，还研究了MICM的热稳定性和化学稳定性。