<br>黑果枸杞多糖和花青素的协同抗肿瘤作用。人结直肠癌LoVo细胞的作用及其分子机制,Food Science & Nutrition

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黑果枸杞多糖和花青素的协同抗肿瘤作用。人结直肠癌LoVo细胞的作用及其分子机制
Food Science & Nutrition ( IF 3.5 ) Pub Date : 2022-04-18 , DOI: 10.1002/fsn3.2892
Xinshu Qin _{1,

2} , Xingyu Wang ₂ , Ke Xu ₃ , Xingbin Yang ₂ , Qing Wang ₁ , Chao Liu ₁ , Xinkun Wang ₁ , Xu Guo ₁ , Jinyue Sun ₁ , Lin Li ₄ , Shiqi Li ₅

Affiliation

黑果枸杞的抗肿瘤作用。多糖（LRPS）和黑果枸杞。本研究对花青素（LRAC）进行了全面研究。 LPRS通过水提取和醇沉淀获得，并使用二乙氨基乙基纤维素(DEAE-Cellulose)和Sephadex G-75柱进一步纯化。使用高效液相色谱 (HPLC) 和傅里叶变换红外 (FT-IR) 光谱对纯化的 LRPS 进行表征。结果表明，纯化后的LRPS中含有杂多糖，主要成分为阿拉伯糖、半乳糖和葡萄糖，重量百分比分别为41.2%、33.6%和10.8%。更重要的是，单独使用LRPS（500μg/ml）和LRAC（80μg/ml）时（孵育48小时）并不能阻止肿瘤细胞的增殖，但当它们一起使用时，发现了显着的抗肿瘤效果，因为LoVo LRPS (150 μg/ml) 和 LRAC (20 μg/ml) 混合物 (LRPS&AC) 在 24 小时内显着抑制细胞（一种典型的人结直肠癌细胞系）。 LRPS 和 LRAC (LRPS&AC) 联合发挥抗肿瘤活性，通过活性氧 (ROS) 依赖性途径阻断细胞周期 G0-G1 期并诱导 LoVo 细胞凋亡。 LRPS&AC的抑制作用对肿瘤细胞具有特异性，不影响正常细胞的增殖。 Western blotting显示，抗肿瘤作用与PI3K/Akt（磷脂酰肌醇3-激酶/蛋白激酶B）和JAK2/STAT3（janus激酶2/信号转导和转录激活子）交叉作用启动的线粒体介导的细胞凋亡有关。 3）信号通路。这些研究结果首次揭示了LRPS与AC的协同抗肿瘤作用及其相关机制，使黑果枸杞得以发挥作用。作为开发具有抗肿瘤特性的治疗试剂和功能性食品的天然来源。

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Synergistic antitumor effects of polysaccharides and anthocyanins from Lycium ruthenicum Murr. on human colorectal carcinoma LoVo cells and the molecular mechanism

The antitumor effects of Lycium ruthenicum Murr. polysaccharides (LRPS) and Lycium ruthenicum Murr. anthocyanins (LRAC) were comprehensively investigated in this study. LPRS was obtained by water extraction and alcohol precipitation and further purified using diethylaminoethyl cellulose (DEAE-Cellulose) and Sephadex G-75 columns. High-performance liquid chromatography (HPLC) and Fourier transform-infrared (FT-IR) spectroscopy were used to characterize the purified LRPS. The results showed that the purified LRPS contained heteropolysaccharides, mainly composed of arabinose, galactose, and glucose with weight percentage of 41.2%, 33.6%, and 10.8%, respectively. More importantly, LRPS (500 μg/ml) and LRAC (80 μg/ml) failed to impede the proliferation of tumor cells when applied solely (48 h incubation), yet remarkable antineoplastic effects were found once they were applied altogether, since the LoVo cells, a typical human colorectal carcinoma cell line, were significantly inhibited by the mixture of LRPS (150 μg/ml) and LRAC (20 μg/ml) (LRPS&AC) in 24 h. The antineoplastic activity resulted from the combination of both LRPS and LRAC (LRPS&AC), by means of blocking the cell cycle at the G0–G1 phase and inducing LoVo cell apoptosis via reactive oxygen species (ROS)-dependent pathway. The inhibitory effects of LRPS&AC were specific to the tumor cells, without imposing on the proliferation of normal cells. Western blotting revealed that the antitumor effect was related to the mitochondria-mediated apoptosis launched by the cross-action of PI3K/Akt (phosphatidylinositol 3-kinase/protein kinase B) and JAK2/STAT3 (janus kinase 2/signal transduction and activator of transcription 3) signaling pathways. These findings for the first time reveal the synergistic antitumor effects of LRPS&AC and the related mechanisms, which enable Lycium ruthenicum Murr. to serve as a natural source to develop therapeutic reagents and functional foods with antineoplastic properties.

更新日期：2022-04-18

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