<br>Cepharanthine 通过调节肠道微生物群改善右旋糖酐硫酸钠诱导的结肠炎,Microbial Biotechnology

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Cepharanthine 通过调节肠道微生物群改善右旋糖酐硫酸钠诱导的结肠炎
Microbial Biotechnology ( IF 4.8 ) Pub Date : 2022-04-19 , DOI: 10.1111/1751-7915.14059
Hong-Gang Wang ₁ , Min-Na Zhang ₁ , Xin Wen ₁ , Le He ₁ , Meng-Hui Zhang ₁ , Jia-Ling Zhang ₁ , Xiao-Zhong Yang ₁

Affiliation

Cepharanthine (CEP) 是从 Stephania Cepharantha Hayata 中分离出来的一种活性生物碱。据报道，CEP的抗炎特性可用于治疗多种疾病。在这项研究中，我们首先发现 CEP 可以改善 DSS 诱发的溃疡性结肠炎 (UC)。通过16S rRNA基因测序、抗生素预处理和粪便微生物移植（FMT）进一步评估CEP对肠道微生物群的影响。结果显示，CEP 处理后，肠道微生物群的丰度显着降低，如Romboutsia 、 Turicibacter和Escherichia-Shigella （尤其是Romboutsia ）。此外，我们通过转录组学与网络药理学相结合的策略探索了 CEP 的机制。转录组数据证实 CEP 通过细胞因子和细胞因子受体途径发挥作用。 CXCL1、CXCL9、CCL7等10个促炎枢纽基因的表达水平与Romboutsia的丰度呈正相关。我们的数据表明Romboutsia是 UC 的潜在病原体。总的来说，我们证实 CEP 通过调节肠道微生物群和促炎细胞因子的表达来缓解结肠炎症。 CEP可以用来设计新的有效的UC治疗策略。

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Cepharanthine ameliorates dextran sulphate sodium-induced colitis through modulating gut microbiota

Cepharanthine (CEP) is an active alkaloid isolated from Stephania Cepharantha Hayata. It is reported that the anti-inflammatory properties of CEP could be employed to treat a variety of diseases. In this study, we first found that CEP ameliorates ulcerative colitis (UC) induced by DSS. The effect of CEP on gut microbiota was further evaluated by 16S rRNA gene sequencing, antibiotic pretreatment and faecal microbiota transplantation (FMT). Results showed that the abundances of gut microbiota, such as Romboutsia, Turicibacter and Escherichia-Shigella (especially Romboutsia), were significantly reduced after CEP treatment. Additionally, we explored the mechanisms of CEP by a strategy integrating transcriptomics with network pharmacology. The transcriptome data confirmed that CEP functioned through cytokine and cytokine receptor pathways. The expression levels of 10 pro-inflammatory hub genes (such as CXCL1, CXCL9, CCL7) were positively correlated with the abundance of Romboutsia. Our data identified Romboutsia as a potential pathobiont in UC. Collectively, we confirmed that CEP relieved colon inflammation by modulating gut microbiota and pro-inflammatory cytokine expression. CEP can be adopted to design novel effective therapeutic strategies for UC.

更新日期：2022-04-19

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